Muridae

Rodent mass datasets discrepancies

I have been looking at weight values for rodents in the family muridae, specifically subfamilies: gerbillinae and deomyinae. I found some considerable discrepancies in the values for the same...

28 June 2016 1,776 5 View

Rodent mass datasets discrepancies

I have been looking at weight values for rodents in the family muridae, specifically subfamilies: gerbillinae and deomyinae. I found some considerable discrepancies in the values for the same...

06 June 2016 1,757 5 View

What can be the reasons for lesser precipitate formation in RNA in situ hybridisation in a PFA fixed & paraffin embedded adult murine tissue?

I am getting very little precipitate in RNA in situ hybridisation for my paraffin embedded adult murine tissue. How can I enhance the purple precipitate in the tissue?

06 June 2013 3,097 6 View

How can I culture purified NK cells in vitro for a long time, for example 1-2 weeks?

I'm culturing murine NK cells in vitro. However, the second day post isolation, 80% or more of the cells are dead or apoptotic. The medium I used was a-MEM supplemented with 10% FBS, 10 ng/ml...

07 April 2013 8,109 8 View

How can I culture purified NK cells in vitro for a long time, for example 1-2 weeks?

I'm culturing murine NK cells in vitro. However, the second day post isolation, 80% or more of the cells are dead or apoptotic. The medium I used was a-MEM supplemented with 10% FBS, 10 ng/ml...

04 April 2013 6,286 9 View

Culturing pDCs from murine bone marrow cells

Does anyone culture pDCs from murine bone marrow cells? I am getting very low yield of pDCs from BM cells. From the hind limbs I get an average of 50 million cells/mouse to start with. I do a 8...

01 January 2013 2,423 6 View

What is the minimum absorbance for a FTIR spectra to be usable in the study?

I am doing ATR-FTIR. I obtain absorbance of about 0.35-0.35. Is it enough? How can I increase it? I was also wondering how many independent replicates are enough for a cell line study in FTIR to...

01 January 2013 3,078 7 View

I've been trying to purify myocytes from adult mice by a percoll gradient to enzymatically isolated cells. It didn't work, any suggestions?

It does not have to include percoll.

10 October 2012 3,169 1 View

siRNA in bone marrow derived macrophages (BMMs)?

We're trying top get siRNAs into murine bone marrow derived macrophages. Any suggestions for protocols and transfection reagents?

08 October 2012 7,597 5 View

No title

01 January 1970 5,757 0 View