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Questions related to Mice
Hello, we want to do methylation study with the Hıgh Resolution Melting Curve (HRM) method. Is there anyone using this method? Is there a study protocol you use? Thank you for your help and support.
10 May 2024 1,499 1 View
Hello! I am trying to analyze some RNAseq data I recently received. I would like to craft a heatmap comparing the expression of specific genes across my cell samples, but I am not sure which of...
09 May 2024 5,636 0 View
Hello everyone. I have a question regarding CFSE proliferation assay with mice. My goal is to see CD3, CD4, and CD8 populations. Why do I keep getting one peak, as if there is no proliferation? I...
09 May 2024 7,719 0 View
Can you explain the pharmacological mechanism of action of dexmedetomidine and how it differs from other sedativesDexmedetomidine: A Comprehensive Review of Pharmacology, Cli...
09 May 2024 2,730 2 View
Hi everyone, I'm going to generate CAR-T cells starting with mouse CD3+ T cells. I isolated mouse CD3+ T cells using Miltenyi Pan T cell isolation kit. I seeded cells 1millon/well in 24 well...
08 May 2024 6,875 3 View
Dear all, When pooling results from multiple ELISAs what is the preferred method of determining the LLOD? I have been using the (mean OD of blank + 2*SD of the blank OD) with individual plates...
08 May 2024 5,281 0 View
Our research is an open-ended questions and we are confused on how to compute given that we do not use rating scale. And if it is needed, what are steps that I need to do? Thank you for your...
08 May 2024 4,532 0 View
some economical statistics only.
08 May 2024 2,542 0 View
Can anyone suggest any kit for SOD activity in plant? I have the protocol for NBT reduction but don't have centrifuge for falcon as the protocol requires 3mL of homogenate to be centrifuged in...
07 May 2024 1,368 4 View
I observe an increase in the PD-L1 expression of CD14+ cells after a special treatment. Now, I want to further analyze the PBMCs for changes in checkpoint protein expressions. I am designing a...
07 May 2024 5,835 2 View
Why is environmental sustainability more important than economic growth and relationship in between economic development and environmental destruction?
07 May 2024 3,603 0 View
Hi, Planning to conduct a few case studies among diabetes participants. Is there any methodology available for the same?
06 May 2024 8,347 2 View
While calculating for K+ where are the cursors supposed to kept at: at the stable traces or near the rise side?
05 May 2024 9,391 1 View
How should healthcare providers monitor patients receiving ivabradine therapy?Ivabradine Therapy in Heart Failure Management: Optimizing O...
05 May 2024 6,053 1 View
What are the medical uses of Atropine?The Role of Atropine in Modern Medicine: Indications, Admini... Atropine has several medical uses across different fields of medicine. Some of the primary...
05 May 2024 1,035 1 View
Swelling of lacrimal gland sack on both sides. Following a long period of dry eyes due to neurological problems and Infractorial Superficial Siderosis
05 May 2024 603 0 View
Hi everyone I produced a batch of virus , enriched it with centrifugation, and titrated it with T cells after 48h. For my experiments I want to use MOI 3 and that's equal to 1/12 dilution. but at...
03 May 2024 9,702 3 View
I want to find example of use of SCAPS-1D for simulating 2D materials based devices. I found a few papers, but they do not specify it in the 2D limit or explicit inclusion of vdW's interaction.
02 May 2024 236 0 View
Hi Everyone, Can I know any available datasets on Retinal Images with Vascular Dementia for a research project. Thank You
02 May 2024 2,722 0 View
I have tried the attached protocol to isolate primary hepatocyte from mice, using Collagenase instead of Liberase. Everything is the same as mentioned in the protocol. Except the following : Used...
01 May 2024 8,815 1 View
Hey, I am wondering which tests I can do to analyze my results and if there is a better way to analyze my results. If I have multiple treatments in columns and I have just one donor in a row,...
01 May 2024 305 2 View
Hi, Does anyone know where to find DNA STRIDER version 1.4? This is the old version that could align different sequences as well as search for oligos or shortersequences in a longer sequence. I...
01 May 2024 797 1 View
In the late 1990s, neuroscientists announced the discovery of the "God spot" in the brain, located in the temporal lobe, just behind the temples. This neural cluster encourages us to ask...
01 May 2024 1,113 4 View
I have Desmond running on my local pc but would like to use the resources of HPC facility. How do I install it on HPC and how to then link it to my pc for using HPC CPU and GPU for speedier...
01 May 2024 6,923 0 View