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Questions related to Mice
I would like to quantify the RNA of cytokines and chemokines from mouse braain tissue, but the availables kits are for proteins essays.
15 February 2022 7,257 2 View
qPCR, capillary sequencer
15 February 2022 2,984 5 View
Ideally, the review I'm looking would encompass infancy through adulthood but anything helps.
14 February 2022 7,027 1 View
Hello We are not getting good responses to either CPP (conditioned place preference) or von Frey with CD-1 mice. We are using morphine at 10mg/kg in 100µL as the positive conditioning but the...
14 February 2022 8,191 2 View
Ideally, the review would start in infancy and go all the way to adulthood.
14 February 2022 3,573 2 View
I will try a new ELISA protocol in my lab, and have to prepare the coating antigen. However, we only have inactived Mhyo cultures for this purpose. Has anyone tried to sonicate inactivated antigen...
14 February 2022 2,353 1 View
I want to dissect inguinal white fat in mouse, but I don't know where exactly it is. And I am confused whether posterior subcutaneous adipose tissue is the same with inguinal adipose tissue.
11 February 2022 6,701 9 View
Hello, I am using Toxtrac for the first time in order to detect the movements of mice while they are habituating in the open field box. Even though the program is fairly easy to use, I am stilll...
11 February 2022 2,838 3 View
I need some papers about the DSS-colitis mice model, where aminosalicylates (as positive control) did not show an effect. Thanks in advance
11 February 2022 6,891 0 View
Could any of you please share a protocol to make Mtb lysates and BCG lysates? I want to make some BCG and Mtb lysates to stimulate mouse splenocytes, Lung and Lymph node cells. So I was looking...
10 February 2022 4,613 2 View
which stage is best to check the negative effect of LPS (Lipopolysaccharides) on the reproductive efficiency of female mice before mating or after mating.
10 February 2022 9,514 3 View
Hi, I'm evoking CA3 schaffer-collaterals and recording from CA1 pyramidal neurons in slice, at a frequency of 20Hz. I've attached two sample traces: one of them has a baseline that has a rolling...
10 February 2022 8,016 0 View
Hi, I'm searching for a fluorescent mitochondria marker (to be used in live confocal microscopy) that is not dependant of membrane potential nor ROS production. I'll have MitoSox to quantify ROS...
10 February 2022 4,310 3 View
Dear All, Have You ever faced problems with atherosclerotic plaque staining? Yesterday I stained mice aortas full of plaques. Very few of them are not stained. So then I repeated the whole...
09 February 2022 8,616 3 View
Experts in the field of metabolism, what is the appropriate fasting period prior to investigating intraperitoneal Glucose or Insulin sensitivity test? This publication:...
08 February 2022 7,123 1 View
Hello, I have been working with H&E staining of mouse muscle (lower limb muscles - gastrocnemius, soleus, tibialis anterior) but seen to have issues with sample preparation. Well, I am...
08 February 2022 7,208 3 View
I need an explanation
07 February 2022 7,223 6 View
An adult patient not ready for General Anesthesia.
07 February 2022 6,690 7 View
Hi all! I want to carry out immunofluorescence to detect macrophages. Is M-CSFR 1 specific to identify macrophages? I am a little bit confused because I have read that this receptor is also...
06 February 2022 8,122 2 View
Hello. I'm currently studying the effect of optogenetic stimulation on social behaviors using the mouse model of autism spectrum disorder (ASD). In one of my experiments, I conducted the...
05 February 2022 5,326 3 View
Dear research fellows, I have to measure interleukin concentration in mouse plasma samples for my master's thesis. My professor would like me to sent some samples to another lab for this...
05 February 2022 5,065 2 View
I have been trying to acquire 2P images during awake and sleep states, however according to the EEG recordings, the mice are not falling asleep. I have recorded around 2-3 h but nothing happens....
04 February 2022 9,511 2 View
I have been wondering why when using a model of high fat diet the control diet has to be the 10% fat instead of normal chow. Does anybody understand the rationale behind this?
01 February 2022 5,585 2 View
Hello, I am planning an experiment to detect the immune response after cytokine stimulation. Does anyone inject cytokine i.v. into a mouse directly and be able to see effect after some time? Thank...
31 January 2022 9,762 2 View