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Questions related to Mice
Has anyone measured the redox potential (Eh) of the DMEM medium supplemented with 10% FBS under standard culture conditions? I need to know this date, I am working with an specie that is dependent...
14 June 2025 3,440 3 View
How does human factors like staffing levels,skill mix and acuity affect patient care/outcomes for the deteriorating patient?
14 June 2025 5,984 2 View
I want to publish my research paper please suggest me
05 June 2025 9,698 1 View
I am trying to write a code to measure current across large time scale (from us up to 1k sec) while the current value is expected to drop from uA down to pA within this time frame. The idea is to...
30 May 2025 5,446 0 View
Collaboration between researchers and policymakers is crucial for developing evidence-based policies that support climate resilience and adaptation in vulnerable communities.
29 May 2025 7,853 2 View
Confusion about the experimental application of FITC-Dextran molecular weight selection: FITC-labeled dextran has good water solubility and fluorescence stability, and is often used as a tracer...
28 May 2025 9,915 1 View
It looks no tissue residual. The lesion current were 400 mA x 2 s. Is the electrode site burned? Can it be restored? See attached photo.
23 May 2025 1,230 0 View
What are the specific steps for using D-luciferin (Luciferin, D-type, bought from MCE; Cat.NO.: HY-12591B) for in vivo imaging? I need to clarify the following key links: Dissolution...
16 May 2025 482 1 View
I understand that in SEC, larger molecules or particles do not enter within in the pores and thereby elute faster with the void volume. However, there certainly have an upper limit of the particle...
13 May 2025 8,638 2 View
Hi, If anyone is looking for a paper review in computer network or cloud computing, I would like to join as a reviewer.
12 May 2025 4,415 3 View
I want to dilute IDT primers for gradient PCR but I don't have Nuclease free water yet. or are there any other suggestions? thank you
08 May 2025 3,913 4 View
Hi All, I am learning to do antibody phage display, and really want to know what value for each steps can be considered as "normal" or "typical", so I can use as a reference when performing the...
07 May 2025 2,965 0 View
Hello everyone, I'm quite new to culturing anaerobic bacteria. I'm feeling both nervous and excited about this new endeavor! Our lab operates on a limited budget, and we currently have access to...
30 April 2025 5,395 0 View
Hello, I am asking if is possible to use flow cytometry cells for an IF. I am new to doing IF. Say I stain my cells for flow cytometry with three colors: fitc, pe-texas red, and dapi. I do this...
30 April 2025 2,767 2 View
In order to reconstitute nucleosomes, I mixed DNA with histone octomers and set them up for a 16-hour dialysis to go from a NaCl concentration of 2 M to 50 mM. I used SnakeSkin Dialysis Tubing at...
30 April 2025 8,819 0 View
The elution buffer is 100 mM Tris(pH=8.0) 150 mM NaCl 1mM EDTA (This is not a metalloprotein) I have adjusted the pH to 8.0. I also have loaded 2 microlitre beads which clearly shows my...
25 April 2025 7,822 2 View
Mother liquor contains DMF solvent and DCC,HOBT as by products.
23 April 2025 1,582 4 View
Recent event in the USA has the world bewildered , where a politician is allowed to destroy the scientific and scholar community without recourse ,even people careers. should all programs be made...
20 April 2025 3,302 1 View
I want to pursue a PhD in labour history to explore how technological automation has transformed the labour force and market in South Asia. Can you recommend some good institutes/faculty or...
14 April 2025 1,810 0 View
I'm investigating the electrochemical behavior of MXenes and would like to know whether they inherently produce redox peaks, even without any target analyte. Any suggestions on how to minimize or...
12 April 2025 2,633 1 View
Hi. I have an isolated small RNA from cancer cells. I want to be sure that there is no contamination with DNA. Does anyone have experienced in agarose gel electrophoresis with small RNA samples?...
09 April 2025 3,888 2 View
Hello all, Thanks in advance for the help. I am simulating the fatigue propagation of an edge-cracked plate. I have the model in N and mm units. I found a reference C and m (C=1.5e-11 MPa.m^0.5...
07 April 2025 2,150 6 View
I've read in a research paper the following: "One of them was the simplified model with a screw without thread, but the thread of screw was simulated by a virtual thread, a function integrated...
07 April 2025 7,800 1 View
Hi, I want to edit a SNP in a non-coding sequence. I can't use the CRISPR HDR approach or prime editing because the PAM or the binding site of gRNA in the ssODN or pegRNA would be changed. I need...
03 April 2025 6,710 0 View