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Questions related to Mice
With ongoing development of real-time "Free Style Libre" insulin detectors, has anyone ever did insulin analysis over a week period for a wild "forest" mice (not wild type lab mice)?
16 August 2022 7,485 4 View
Stability of human serum insulin
16 August 2022 4,984 1 View
I need to administer letrozole and/or dutasteride to mice via i.p. injection. I found papers dissolving dutasteride in saline + 0.3% gelatin, which I added at 1.25mg/ml. I found another paper...
16 August 2022 3,370 0 View
My study needs to test the adipose tissue derived stem cells' effect on the fibroblasts. I planned to perform a indirect co-culture of these two kinds of cell through seeding ADSCs in the 0.4 um...
15 August 2022 3,003 3 View
Effect of gold nanoparticles of different diamaters(10, 29, 50 nm) on blood serum levels for albumin , SOD ,CAT, GPx , cardiac enzymes
15 August 2022 7,463 0 View
Hellow I am performing illumina mini seq of 18 TB targeted gene. So when i amplified my targeted gene,i have to add internal control with master mix. But i am not clear about the appropiate...
15 August 2022 7,164 3 View
Dear proteomic experts, I just recently noticed on Metascape ''Note: gene lists containing more than 3000 genes are not analyzed. Please use a more stringent hit calling criterion, as you most...
14 August 2022 9,891 0 View
Dear friends, if we want to use a human tissue sample preserved in formalin for acid digestion, at what temperature and for how long should we dry it?
13 August 2022 8,077 0 View
Hi, recently I tried to express my genes (from plant) into Rosetta De3 pLysS using this protocol: 1. 1% overnight culture into a new media + antibiotic, incubated to grow until OD600= 0.5 2....
11 August 2022 2,572 1 View
I have already tested several forms of purification to obtain the monomer AB peptide, all of which have failed, it has aggregated quickly, I only obtain oligomers.
10 August 2022 136 1 View
I am wondering if a (semi) solid substrate/scaffold (could be a hydrogel) is necessary for endothelial cells to sprout out to a VEGF producing source. In other words, it it possible to have...
08 August 2022 3,217 2 View
I isolated E.coli (oyster sample) from EC mug to EMB plates. I don’t know what happened to my other plate. The one with metallic green sheen is the positive control while the other plate is from...
07 August 2022 1,044 3 View
Hello Folks, I have been using the Neurite Tracer for measuring the lengths of the dendrites of B16F10 cells treated with some melanogenic compound. The actin filaments were stained with...
06 August 2022 8,011 0 View
Who else here is using the PiMP platform (designed by Glasgow Polyomics, UK) to analyze their metabolomics data? I used this platform and extracted the data in a .csv format. Then I loaded them on...
04 August 2022 6,276 3 View
I need to differentiate among various stages of spermatogenesis along with the differentiation of the immature and mature Sertoli cells. Also if you can put your valuable suggestions with regard...
03 August 2022 2,764 3 View
Hi guys, I have 27 mer siRNA duplexes from Origene, and the company didn't provide the Mwt. I tried to search but no luck so far How can I calulate it or find it?
03 August 2022 6,965 2 View
I am doing research of osteoclast. What kind of cell line is using for osteoclast studying except the Raw cells and BMM cells? Thank you for give me sUggestion
02 August 2022 1,625 1 View
Generally a type of polymerase which is Taq polymerase which works nearly at 60-70 degree centigrade,but whereas are there any polymerases which work above 95 degree centigrade?
28 July 2022 2,567 3 View
Looking for tips to optimise the IHC staining of PAX-2
28 July 2022 4,856 0 View
We have a gene with two isoforms, one with a longer 5' UTR and one with a shorter 5' UTR. It's been demonstrated that which isoform is predominant changes over development. We want to study how...
28 July 2022 2,303 2 View
Is it possible to encapsulate both i.e. peptide antigen and TLR 7 agonist (Imiquimod) together in a single SLNPs?
24 July 2022 1,658 3 View
I have Creality Ender 3 Max 3D printer. Please share the 3D printer STL file with me. It will help me to print . Beginner level.
22 July 2022 7,606 3 View
I am studying a chronic condition (diabetes) in human samples and preliminary ex-vivo results show a difference in cytokines secretion. I would like to do ICS to define the subpopulations that...
21 July 2022 9,210 0 View
I want extraction RNA from tissue in which ocurre blood clots due to DIC. Which way will be the best to removing blood clots from tissues? Dipping a tissue in sterile PBS? Or however perfusion of...
21 July 2022 330 0 View