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Questions related to Mice
how is prion can cause disease
02 August 2024 525 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
02 August 2024 2,525 0 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
02 August 2024 645 2 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
02 August 2024 9,198 10 View
I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...
02 August 2024 8,653 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
01 August 2024 4,903 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
01 August 2024 4,141 0 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
31 July 2024 3,720 0 View
I am looking for a published article using SAS or SPSS Generalized linear model for trial/event data and not survival analysis. Both software packages off the option for the number of success out...
31 July 2024 3,972 2 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
31 July 2024 2,478 7 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
31 July 2024 7,124 0 View
I am beginning to use OG+ in my district and noticed during training there are a lot of materials and routines, which may be overwhelming for my students, especially those on the autism spectrum....
31 July 2024 5,930 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
31 July 2024 1,155 2 View
i am comparing the growth performances of filamentous and non filamentous forms. in a case where I can not count the cells of the filamentous algae using a hemocytometer, is it okay to do a...
31 July 2024 6,013 2 View
Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.
30 July 2024 6,742 3 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
30 July 2024 6,666 5 View
I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...
30 July 2024 288 2 View
After a lot of feature engineering for click-through rate modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing it?
30 July 2024 5,366 0 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
27 July 2024 2,145 0 View
I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?
27 July 2024 5,062 1 View
I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.
26 July 2024 6,639 2 View
While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...
26 July 2024 9,467 1 View
I have designed magnetic material with different biasing conditions in HFSS. Now I want to give an RF AC signal and do a transient simulation in HFSS. Is it possible to do in HFSS? Please help me...
25 July 2024 9,422 5 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
24 July 2024 2,974 1 View