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heavy metals from powder particles of roots, flowers and seeds
30 April 2024 9,162 5 View
The Gibco DMEM with which I have been working for quite some time is turning yellow while it is in 4 degC and also has froth. A similar medium was prepared with FBS earlier than this one and it is...
30 April 2024 3,141 0 View
Please how can I get the cracked version of SIMCA for PCA analysis?
30 April 2024 2,018 11 View
Can anyone share their experience regarding the acquisition or gifting of cell lines mentioned in a Nature article? Specifically, I am interested in knowing if there are any journal-specific...
30 April 2024 891 4 View
Dear Jose, Hi, are you working or retired. I still work as the professor at BSUIR. My mail [email protected]. Prof. Vishniakou, Minsk.
28 April 2024 3,414 1 View
Please is there anyone who has a tutorial video on metaboanalyst 6.0 or who can assist me to run it on my LCMS/GCMS Data for untargetted metabolomics studies on soil and plants? We can collaborate...
28 April 2024 566 1 View
I use 7AAD and ANNEXIN-V-FITC to detect cell apoptosis on a flow cytometer. I have some questions I would like to ask you. Regarding the FSC and SSC gates, should the group of cells on the upper...
27 April 2024 4,210 3 View
How to calculate IC50 for different populations have anticancer activity
27 April 2024 5,049 3 View
I am writing a case report on a rapidly growing symptomatic brain tumor. The patient had an initial brain MRI which was unremarkable, and no brain tumor identified. after 47 days the patient was...
27 April 2024 6,840 0 View
Hi, I would like to ask for a help from the Research Gate community regarding a statistical analysis. My case is analyzing the association between the concentration of HIV DNA prior to therapy...
26 April 2024 7,680 0 View
Dear Community, I am investigating the subcellular localizations of some proteins in HEK293T cells and need to stain lysosomes as a spatial reference at the same time. Is there any good antibody...
26 April 2024 8,265 2 View
I am planning to do immunofluorescence (IF) and immunohistochemistry (IHC) from rodent tissues. Is it fine to perform IF and IHC from tissue sections from another tissue of same animal group that...
26 April 2024 449 2 View
Hello everyone, I am currently growing Human microglia cells HMC3 in 100 mm plate. My question is what is the estimated Protein concentration (within range) of 1 ml HMC3 cell lysate? I usually...
26 April 2024 4,570 3 View
Hi there, adding a paper or towel to the tlc chamber is told to fasten the chamber saturation in TLC. But i could not find any information what paper can best be used for that - which works/which...
25 April 2024 7,474 4 View
The Campinha Bacote Model of Cultural Competency fosters awareness, desire, knowledge, and skill by encouraging recognition of biases, promoting a commitment to understanding diverse cultures,...
24 April 2024 3,614 0 View
I am staining mouse brain tissue sections using an anti-GFAP primary antibody (for astrocytes). The results are coming out pretty weird. Some sections have decent staining, and some others have...
24 April 2024 4,149 1 View
Can you find a food chain without producers why and energy transformed and transferred as it flows through the food chain?
24 April 2024 5,591 0 View
I plan to conduct an experiment in which conditioned medium of MSCs will be used as a therapeutic agent. Any idea how I can concentrate the medium before using it for treatment. Thanks.
23 April 2024 978 3 View
Impact of various socio-economic factors on vaccination rates among children living in urban settings, both in the USA and Central Asian nations.
23 April 2024 5,183 0 View
Metabolic disorders occur due to our proper habits of eating process and remineralisation in the mouth. Like food, water, and liquids, etc. Due to mutations in all our food in saliva, the...
23 April 2024 1,935 3 View
Hi, I would like to perform the DHE assay to test antioxidant compounds on HT29. I am using antimycin A (100 microM) as a positive control (mitochondrial chain inhibitor). I have a good response...
23 April 2024 9,402 4 View
I am reaching out regarding the issue of conversion devices/auto sears/switches that make it possible to convert semi automatic pistols and rifles into full automatic. These devices are...
20 April 2024 8,784 0 View
Why is pyramid of biomass better than pyramid of numbers and difference between a pyramid of biomass and a pyramid of numbers?
20 April 2024 9,295 2 View
The question explores the impact of Brucellosis in packed milk on public health and seeks innovative methods for detecting and reducing this risk in dairy products.
19 April 2024 3,706 0 View