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Questions related to Mice
let me first explain my SDS gel, the left 4 lanes ( Lane 1 to 4 ) contain individuals proteins. the right 4 lanes (lane 5 to 8) contain a mixture of the individual proteins with different buffers....
31 January 2022 1,314 0 View
As more number of major and lengthy surgeries are done laparoscopically. I'm looking for some clear-cut guidelines or recommendations in this regard especially degree of head down tilt allowed or...
31 January 2022 2,075 7 View
I am looking for experts in the field of perioperative hypothermia for a content validation study. I am very happy if you support me here! The inclusion criteria are: Certified nurse with at...
28 January 2022 5,076 3 View
With the newly characterized phenomenon of load-dependent based indices of HFpEF and HFrEF, and many more, is ejection fraction (EF) the proper index to be used to characterize failing cardiac...
27 January 2022 1,959 6 View
Dear colleagues, I have applied 1 ug/mL of doxycyline to human iPSCs but most cells died within 24 hours after treatment. I was wondering if you have applied doxycyclin to iPSCs and know what...
27 January 2022 1,411 1 View
I have a specific Huntingtin mutant liver model and when I perfuse the liver via the portal vein, in order to dissociate and plate out primary hepatocytes, the Glisson's capsule bubbles up as if...
27 January 2022 512 1 View
In my research, I often need to make frozen sections of mouse hearts, but I found that the position of the heart was not constant during the embedding process due to the irregular shape of mice....
26 January 2022 7,181 1 View
I have dissolved Docetaxel in DMSO to a concentration 0.5mg/ml, from which I take 1ul and dilute in saline (1:100) for mice injections. When I dilute docetaxel which is already dissolved...
26 January 2022 4,728 1 View
I'm injecting microsphere beads for particle counting using a syringe. To secure reproducible measurement results, the sedimentation should be minimized. Any good way to do this? Increasing...
25 January 2022 5,380 4 View
I use the ultracentrifugation protocol to isolate exosomes from cell in culture (BV-2 microglia cell line). I was wondering if there is a viable protocol to isolate specifically microglia-derived...
24 January 2022 6,753 1 View
Hello everyone I inoculated C57BL/6 mice with TRAMPC2. I saw ulceration after 32 days of inoculation. anyone knows why it happens? except of injection manner, is there another factor to make this...
23 January 2022 2,592 3 View
Thanks to anyone how would like to spare me attention first. I am working on treating primary mouse hepatocyte with drugs may cause dyslipidemia, hoping to find any positive results on their...
22 January 2022 9,614 1 View
I needed a frozen section of the salamander's heart for immunofluorescence staining, but unfortunately I found that the heart was too loose for me to get a good shape. I wonder if there is any way...
21 January 2022 7,495 1 View
I extracted my brain organoid samples recently and ran them on SDS-PAGE. These brain organoid samples were coated with Matrigel, I tried to remove as many as I can by washing them in cold 1X PBS...
21 January 2022 8,541 4 View
I am trying to measure microglia activation in response to an insult with qPCR. I have used Iba1 and found no differences in the expression between my groups at different timepoints. I am using a...
20 January 2022 5,834 2 View
I am piloting prenatal ethanol exposure in mice and I need a way to measure blood alcohol concentration. What are the best enzymatic/colorimetric assays to use? Looking for something simple and...
19 January 2022 6,030 2 View
Hi! I am trying to polarize RAW 264.7 macrophages on the 7th pass (ATCC) with 100 ng/mL LPS (Sigma) for M1 and 40 ng/mL IL4 (Sigma) for M2. They were plated at a density of 20,000 cells per well,...
19 January 2022 4,637 4 View
During my internship, I fixated chondrogenic cell pellets in 1ml of 4% PFA. The first time, an incubation period of 1 day acoording to the lab's protocol. The second time, due to holidays I...
19 January 2022 8,217 3 View
Hi everyone. I'm having problem with quantifying viral reads from my RNA-seq data, which is part of my Final Year Project. Basically, I have filtered the host reads by aligning to host organism...
19 January 2022 3,984 3 View
What concentration of camptothecin is effective to kill mouse fibroblast in 24h?
18 January 2022 5,295 2 View
thanks for answering
18 January 2022 9,404 1 View
Can I pool mouse blood from different individuals of the same strain without regard to any factors? Or do I need to consider something like mouse blood types etc.?
18 January 2022 7,376 3 View
Im interested in studying the effects of neurotoxins in neurotransmitter (dopamine,Gaba,serotonin, acetylcholine)release in mice brain.
17 January 2022 5,003 2 View
Hi everyone! I'm trying to detect proteins of ~110-150kDa in mouse heart by western blot. e.g. SERCA2a, Apaf-1...I realized I always see 4 unspecific bands starting from ~75kDa to 150kDa,...
17 January 2022 1,470 3 View