I am not an expert in this field, but I am very interested and have researched to find an answer. I received some assistance from tlooto.com for this response. Could you please review the response below to see if it is correct?

Crude extraction using NaOH and Tris can be effective for fungal DNA extraction but may not be optimal for all species due to the robustness of fungal cell walls [1][4]. An improved method involves incorporating a simple lysis buffer, such as SDS or CTAB, combined with mechanical disruption like bead-beating or vigorous mixing, which helps break down cell walls and release DNA without heating [2][5]. Subsequently, DNA can be precipitated with isopropanol or ethanol and purified through washing steps [3]. This method balances simplicity and effectiveness, making it suitable for fungal DNA extraction in resource-limited settings [1][3][4].

Reference

[1] Chi, M., Park, S., & Lee, Y. (2009). A Quick and Safe Method for Fungal DNA Extraction. Plant Pathology Journal, 25, 108-111.

[2] Osmundson, T., Eyre, C., Hayden, K. M., Dhillon, J., & Garbelotto, M. (2013). Back to basics: an evaluation of NaOH and alternative rapid DNA extraction protocols for DNA barcoding, genotyping, and disease diagnostics from fungal and oomycete samples. Molecular Ecology Resources, 13.

[3] Płaza, G., Upchurch, R., Brigmon, R., Whitman, W., & Ulfig, K. (2004). Rapid DNA Extraction for Screening Soil Filamentous Fungi Using PCR Amplification. Polish Journal of Environmental Studies, 13.

[4] Yang, Y., Zuzak, K., & Feng, J. (2016). An improved simple method for DNA extraction from fungal mycelia. Canadian Journal of Plant Pathology, 38, 476 - 482.

[5] Lahuf, A., Jaafar, O., & Hameed, Z. (2019). A simple, rapid, safe and low-cost method to extract DNA from phytopathogenic fungi.. Asian Journal of Agriculture and Biology, 7, 197-203.

Glass bead pulverization with vortexing seems to be the best method according to this paper:

Article Comparison of six extraction techniques for isolation of DNA...