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Questions related to Mice
Hi there! I'm trying to perform an experiment to prove one specific protein interacts and activates NMDAr. For this purpose i'm trying to use Fura-2AM dye to see changes in Ca2+ levels inside the...
07 June 2022 4,972 1 View
I am recording sEPSC(holding at -70 mV) and sIPSC(holding at 0) these days. My internal solution is Cs-based low Cl solution. But EPSC still appears when I was recording sIPSC which is obviously...
06 June 2022 9,004 3 View
I want to find the protocol for in vitro efferocytosis assay, but I found that almost all the research is based on the macrophage "eat" Jurkat cell debris. Why are we using the Jurkat cell in...
06 June 2022 4,087 1 View
Hi all, We are trying to recombine a WT gene with mutated gene in Klebsiella pneumoniae chromosome. We are using a recombineering method described by Li et al (ref:...
03 June 2022 3,003 0 View
While performing a Fluorospot assay, generally there should not be a situation where non-specific spots are observed. However, after performing the experiment multiple times by multiple people in...
03 June 2022 9,446 0 View
Hi, good morning. I want to ask if some of you have experience in isolating bone marrow cells from fetal mice? Is it suitable to use the same method as adult mice such as flushing technique for...
02 June 2022 4,155 1 View
The mechanism
02 June 2022 3,617 6 View
Weils stain for staining myelin.
02 June 2022 9,506 3 View
Hello, I intend to perform thermal unfolding of my proteins by CD, to determine their melting temperatures. I initially scanned the proteins over a wavelength range of 250 -190 nm at 25 °C (in...
01 June 2022 116 5 View
Facial vein blood withdrawal is difficult for irradiated and old mice
31 May 2022 2,225 1 View
Hello, We had a discussion on whether the leukocyte population can shift between different samples on the FSC / SSC plot and should the gate be adjusted on individual samples therefore? We are...
31 May 2022 4,491 1 View
Hi, I wonder if anyone knows how to create ensembles using the pdb-tools (https://wenmr.science.uu.nl/pdbtools/reference)? The protein-protein docking have been completed by InterEvDock2 where I...
31 May 2022 621 1 View
Hello, I would like to ask whose are studying about sonication of the alpha-synuclein preformed fibrils. The preformed fibrils are commercially available but in order to use for disease models,...
30 May 2022 174 3 View
When trying to precondition/priming MSC after seeding 4000 cells/cm2 , do we need to wait until the cell attach to culture plate to add cytokine such as IL-17 or do we add the cytokine with our...
27 May 2022 2,416 0 View
Dear All, I am doing a meta-analysis for which I have used GraphPad software for analysis. I'm facing difficu8in certain domains of analysis. I would be delighted to receive opinion of the house
27 May 2022 2,858 0 View
Spiking the sample with 1microgm/ml and sample volume was made 50ml. After doing solid phase extraction elution was done with 2ml of eluent and analysis was done with HPLC
26 May 2022 5,977 4 View
We use the same backbone and helper plasmid for both, and the only difference is the Rep-cap plasmid 2/6 vs 2/9 from addgene. We titer by qPCR with a standard curve. Is this a known phenomenon and...
24 May 2022 6,640 0 View
Can anyone tell me is this a contamination or just a cell changing its morphology?????
23 May 2022 6,841 4 View
Hi, I need to differentiate osteoclasts from the bone marrow of mice, I would really appreciate if someone can share the protocol with me? I usually differentiate macrophages from mouse bone...
18 May 2022 1,378 0 View
Hi guys. Recently I had read an article, and I’m quite puzzled about the results of the quantifications of immunostaining. How did the percentage of immunoreactive positive area was bigger than...
18 May 2022 665 2 View
I've been cleaning with bleach and distilled water, but it's still very difficult to get it completely clean. I'm wondering if anyone can recommend a detergent?
18 May 2022 1,890 1 View
Hi, I pulled out mouse embryonic stem cells from -80 (where they have been for a week when I froze them down) and plated them on gelatin plates without feeders. The next day I observe these round...
17 May 2022 1,670 3 View
I am using the Fos2Trap mice crossed with a Ai14 to see dTomato in fos cells activated by 4OH tamoxifen. The dTomation expression is nice on untreated slices but as soon as I perform an...
17 May 2022 7,473 4 View
Good evening, so my question is about chemilumiscence. So when I use a rabbit primary antibody for.my phosphorylated protein, put the rb-HRP 2. antibody on it and envelope it with ECL-solution,...
17 May 2022 7,462 1 View