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What causes artifact in TH Nissl IHC staining mouse tissue?

Hi, In our lab we have been performing perfusions and immunohistochemical stainings for the dopaminergic marker TH combined with Nissl, on mouse brain tissue for many years. Once in a while, we...

15 January 2025 4,473 0 View

Do I need to charcoal-strip the FBS?

Hi everyone. We have been using charcoal-stripped FBS in our lab for many years now but are considering switching to non-stripped FBS and stripping it ourselves because it is significantly...

14 January 2025 6,910 1 View

How do IoT devices handle power management and energy harvesting?

Device and Hardware

13 January 2025 9,341 3 View

Journal free publish Q1?

hello. I need help please tell me one journal with no fee pay with Q1 in subject drug resistance and another in genetic similarity, please agency reply, I will try to submit.

12 January 2025 2,481 0 View

Patent filed for ultrasound-based teeth whitening technology?

Patent filed for ultrasound-based teeth whitening technology

10 January 2025 7,574 4 View

What are the impacts of the clinical nursing evaluation on the complications of a vascular hemodialysis access?

The importance of the nursing role in the clinical evaluation of arteriovenous fistula in hemodialysis

10 January 2025 5,914 2 View

How can the rehydration buffer of 2-D electrophoresis be avoided from crystallization?

I have prepared a stock of rehydration buffer containing 7 M urea, 2 M thiourea, 4% CHAPS, 1.5% SB-12, 65 mM DTT, 0.1% protease inhibitor cocktail, and 0.1% Ampholyte. Then, I aliquoted the...

08 January 2025 4,435 1 View

What is the most important problem raised by the issue of criminal responsibility for media crimes?

By media crimes we mean: print, audio-visual, and electronic media.

08 January 2025 10,069 0 View

Are there signs of a resurgence of COVID-19?

Symptoms of COVID-19 may appear, but in different forms than the original ones. What are these symptoms in your country, and how can they be diagnosed? What are the basics of treatment?

30 December 2024 1,226 0 View

How can I narrow than a research topic which is related to formative assessment in TEFL?

It will be a quantitative research. (For MA. thesis) in the context of Iran.

27 December 2024 4,977 2 View

What kind of Membrane do i need for dialysis of recombinant TNF-alpha?

Good afternoon. I am writing a scientific paper on the production of recombinant TNF-alpha. I am focusing on the downstream process. The steps are two-step ion exchange chromatography. And then...

24 December 2024 7,231 3 View

Why do I not have a spot in my SDS-PAGE after IEF?

I do a 2-dimensional electrophoresis for a human tissue. My steps were as follows: Whole protein extraction from 4 samples by grinding tissue in liquid nitrogen and solving proteins in rehydration...

24 December 2024 1,546 4 View

Why we multipling sample size(Design effect) in cluster sampling?

why we use design effect in cluster sampling

23 December 2024 6,827 3 View

Which Tools Should I Use to Identify Activated or Suppressed Pathways from qPCR Data?

I’ve got gene expression data from qPCR, and I’m looking to identify which biological pathways are activated or suppressed in my cell line. I’ve already sorted out the fold change values and...

18 December 2024 6,687 2 View

How differentiate THP1 cells to M2 macrophages?

I am differentiating THP1 cells to M2 macrophages using PMA anf IL-4 and IL-13. For THP1 cells I have used RPMI@ATCC media with 10% FBS, 1% anti-anti and 0.05mM beta marcaptoethanol. I have tried...

18 December 2024 8,970 2 View

How can I calculate Number Mean Diameter using the Discrete method in ANSYS Fluent's Population balance model?

I'm working on particle synthesis and for that I need to calculate Number Mean Diamteer, I require steps to calculate and visualize the Number Mean Diameter using the Discrete method in PBM ANSYS...

14 December 2024 4,500 3 View

Purifying co-complex protein ?

i am trying to purify co-complex protein. two plasmid has been transformed in a single e coli (Rosetta com cell ). Then did primary and secondary culture induced IPTG ( 0.25mM) at 0.5 OD (600nM)....

12 December 2024 7,724 3 View

I need research papers on adaptive modulation and coding for a 5G sysytem?

i need research papers on adaptive modulation and coding for a 5G sysytem

10 December 2024 4,475 1 View

How do I prevent my peptide from sticking to my yeast surface?

I have engineered my yeast to display a molecule, which should selectively modify my endogenously introduced peptide. At the end of the reaction, I centrifuged the yeast cells in the media,...

05 December 2024 2,616 0 View

Does adding media to Suspension cells( e.g. THP1) is considered an increased passage number?

For THP1 or any suspension cells, is it that no matter how much more media(even if it's twice or more than the original media) I add without taking the cells and centrifuging them, it's still the...

05 December 2024 2,616 0 View

Cell membrane dye DIR-labled cells during live imaging?

Hello everyone, I need help. I have a question concerning the cell membrane dye DIR for cell infusion during live imaging. rescently, we want to evaluate the ability of microglia to cross blood...

29 November 2024 9,873 0 View

Are you the copyright owner of the following graphics?

Dear Mr. Wu, I have a question: Are you the copyright owner of the following graphics?...

29 November 2024 1,329 2 View

Qz source inverter ?

Why does the experimental boost voltage factor of my QZ Source Inverter, using a straightforward control system, reach only 2.5, while the simulation results show a boost factor of 4 to 6?...

28 November 2024 870 4 View

How do I remove the name of an author who I mistakenly accepted here on ResearchGate as a co-author of an article?

I mistakenly accepted an author as a co-author but who did not co-author the article with me.

27 November 2024 4,285 0 View

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