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Questions related to Mice
I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...
17 July 2024 7,444 1 View
Theoretical framework
16 July 2024 1,465 1 View
Dear sir/Madam, I am Parinaz Parsa the first writer of article "Optimisation of probiotic yoghurt production enriched with phytosterols" and understand that one person is mentioned as writer which...
15 July 2024 9,543 2 View
So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...
15 July 2024 611 1 View
Hi, I have performed an Annexin V apoptosis study using flow cytometry and got quite good results. I doubt how to represent the quadrant percentages in the final bar graphs. Some papers only show...
14 July 2024 310 3 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
14 July 2024 1,632 3 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
14 July 2024 7,420 1 View
How do gradient and discharge change in a downstream direction and why are glacial valleys shaped differently from river valleys?
14 July 2024 9,183 3 View
a man was facing a paralysis problem.. he wants some drink or else food not even he ask, because he paralyzed .while he put the smart spectacles he blinks the eyes it will count the sensors and...
14 July 2024 7,983 1 View
we made an IPMC and we want to store it in the best conditions possible. what do you suggest?
14 July 2024 9,619 2 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
14 July 2024 9,912 3 View
Hello, Our lab has been experiencing sporadic issues with coverslipping. We use the Sakira Tissue Tek Prisma H&E Stainer and a Tissue Tek Glas2 Automated Glass Cover slipper. Periodically,...
13 July 2024 6,964 3 View
I want to know the best methods to automate my job and live without working?
13 July 2024 5,895 2 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
12 July 2024 5,336 0 View
Here is an opening in our lab for a post-doctoral fellow position. Please help me to circulate the...
12 July 2024 2,435 2 View
need to know protocol for photoplate creation, styles, etc
12 July 2024 8,280 2 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
12 July 2024 8,437 0 View
General mistakes which could happen during DNA isolation of plants
11 July 2024 9,443 3 View
I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...
11 July 2024 7,101 2 View
Hi, I'm a graduate student, college of nursing, chungnam national university in Daejeon. The topic of my thesis is "Quality Assessment of Tools for Evaluating Acute Postoperative Pain." I have...
11 July 2024 3,012 1 View
This research question explores the complex relationship between bacterial diversity in the oral microenvironment and the clinical manifestations of periodontal disease. The oral microenvironment...
10 July 2024 6,172 1 View
This research question explores the complex chain of relationships of how the oral microecology system affects various aspects of oral health and further affects systemic health. The oral...
10 July 2024 3,871 0 View
My attempt to promote listening was "Using Short-stories". It works fairly but there could be better options. Role of Short Stories in Developing English Listening Skill
10 July 2024 9,032 4 View
Hello everyone, We are trying to differentiate the KOLF-NGN2 hiPSC into neurons - A reference human induced pluripotent stem cell line for lar... . The differentiation is characterized by 2...
10 July 2024 3,964 1 View