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Questions related to Mice
In an upcoming in vivo experiment, we will be administering enzalutamide by i.p. injection in nude mice. We plan to dissolve Enzalutamide in DMSO, and then dilute this solution using normal...
17 May 2022 8,836 3 View
Hi, Im trying to isolate intratestinal macrophages from mouse lungs, and grow them in culture for a few days, to collect secretome. Does somebody have a good protocol for it? I tried face...
12 May 2022 994 3 View
I need to know which type of amyloid beta protein (human or rat/mouse) can be injected in mouse through ICV surgery for induction of Alzheimer's like phenotype.
12 May 2022 7,630 1 View
Is there any research on the mental health status of pre- and post-doctoral researchers and/or academic staff with samples from Germany? I have a hard time finding papers on the topic, so if you...
12 May 2022 4,465 3 View
Hi everyone, Been trying to culture murine T cells isolated from spleen or lymph nodes (iLNs) but been having difficulty in keeping the Pan T cells alive even after only 24hr of culturing...
12 May 2022 5,766 2 View
I'm currently doing IHC (diaminobenzidine method). If I couldn't cut the brain right before it sink in the sucrose, how is the best way to preserve the brain signal? Is it a good idea freeze the...
07 May 2022 3,743 0 View
Hello all, I have serially diluted mice fecal sample and plated -1, -3, -5, -7 dilutions in the selective medium (E.coli). In -5, -7 dilutions, no growth were seen. But in -3 and -1 dilution, I...
06 May 2022 2,811 1 View
I'm doing immunofluorescence imaging on frozen sections of guinea pig and pig cochleae. The antibodies that I'm using are anti-CTGF to stain type 4 fibrocytes and anti-AQP1 to stain type 3...
06 May 2022 998 3 View
Hi all, I am trying to reduce leukocyte contamination from my platelet-rich plasma sample from a dog. If anyone has any experience in using mouse anti-CD45 microbeads for leukoreduction in a...
03 May 2022 7,161 1 View
Hello everybody My research group and I are trying to do an immunofluorescence for NOGO-A protein, but we are not getting the markings right. For the technique, we used antigen retrieval with...
03 May 2022 8,419 0 View
Hello, I am currently doing a Human Transferrin Receptor ELISA https://www.abcam.com/human-transferrin-receptor-elisa-kitcd71-ab272469.html. When doing the standard I didn't make the right...
29 April 2022 1,233 3 View
Would really appreciate it if anyone can share some insights on which one is easier to culture, has more reliable insulin secretion and etc.
28 April 2022 4,451 4 View
I recently stained with a rabbit antibody and tyramide boost, and now want to add another rabbit anitbody requiring a tyramide boost (different secondary channel). I am using a microwave treatment...
27 April 2022 2,468 1 View
This is the result of immunohistochemical staining using paraffin-embedded mouse sections. Dewaxing, hydration, antigen activation (ph6.0 citrate, autoclave), blocking, and incubation of primary...
26 April 2022 5,091 1 View
Dear fellow knowledge seekers. I have a possibly quick question to ask to the IHC experts in here. Following scenario, I have a couple of unperfused snap frozen brain tumors and I'd like to...
25 April 2022 6,184 3 View
I am looking for good GAD1(glutamate decarboxylase 1) primary antibody for immunofluorescence staining of cortical areas in mouse brain slices. It would be best if soma in which expressed GAD1...
24 April 2022 8,515 1 View
I am working on the anti-corrosive application of green inhibitors. I have received a reviewer's comment that current overload may be seen in the polarization curve. How do I identify it from the...
22 April 2022 6,402 7 View
I plated PC12 cells in 6 well plate, and now I am trying to prime the cells with 50ng/ml of NGF. After incubation for 11 days, do the cells express more TrkA compared to the unprimed ones?
22 April 2022 747 1 View
Can we use FeCl3 instead of FeCl3. 6H2O in FRAP assay?
21 April 2022 1,783 5 View
Questionnaires or surveys done regarding any illness or condition would be helpful. Specifically treatment awareness for cerebral palsy or such disability.
21 April 2022 6,200 8 View
Hello, I would like to do qRT-PCR to look for a specific gene in a human metastasis in mouse brain samples, so I need a sequence that only recognise human gene and doesn't cross with mouse genome....
20 April 2022 4,203 2 View
A common negative control setup in immunofluorescence is the omission of primary antibodies. What purpose does a negative control serve in these contexts? Is it just to get an idea of the...
20 April 2022 4,021 3 View
Hi, My lab is beginning to delve into immunological assays, and I am going to run some indirect ELISAs using serum IgG collected from mice in an immunization study. I'm a bit confused on how to...
19 April 2022 9,954 4 View
I am trying to culture primary microglia cells from adult mice but for some reason I am getting really small cells and not seeing the typical morphology of microglia cells. Even when cells are put...
18 April 2022 7,472 2 View