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I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...
06 June 2024 8,212 0 View
Antiplatelet Therapy: Mechanisms, Indications, Administration Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?
06 June 2024 6,478 1 View
Which of the staining method (crystal violet/ hematoxylin) is preferred to stain the trans well migration assay membrane and why?
05 June 2024 2,787 0 View
Hello everyone! My PI would like me to measure the amount of mitochondrial DNA in multiple cell lines using qPCR targeting COXI and COXIV. However, I have never done a qPCR that measures...
04 June 2024 6,820 1 View
I've been having some difficulties with my western blots, but this time, a new issue arose. Do you have any idea why I may be getting these vertical lines? Or if you have any other...
04 June 2024 7,500 0 View
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04 June 2024 7,231 3 View
Hi, I am confused with the calculation regarding the BOD5 for seeded dilutions using the below formula BOD mg/l = (D1-D2)-(S)Vs/P (As per standard methods). Could any one of you please explain...
04 June 2024 4,755 2 View
The question asks about the timeframe for running gel electrophoresis after nuclease-free water has been added to DNA samples.
04 June 2024 1,656 3 View
blue tea from butterfly pea plant
04 June 2024 2,580 1 View
I am currently running Cell Death ELISAs, using the Roche Cell Death Detection ELISA kit. My samples are from the supernatant of bacterially infected primary canine cells. I am seeing no color...
03 June 2024 8,709 1 View
I would like to look at mitochondrial density in transverse sections of intestine. I do not have access to a TEM. I cannot really determine based on an internet search whether it is feasible to do...
03 June 2024 5,389 2 View
Hi,I have been trying to transfect MDA MB 231 cell lines since a while, I tried with lipofectamine LTX as well as lipofectamine 3000.Please suggest how to proceed with this cell line and what is...
03 June 2024 1,596 0 View
Hi all, so I did western blot to detect GAPDH as my control. Despite repeating three times, all my western blot results shows variability in different aspects. I've tried to use commercial...
03 June 2024 9,338 1 View
I'm doing a 3C analysis shown in the picture linked and attached below with the Bait fragments labeled and the target fragments that will be tested for interaction with the bait labeled with...
03 June 2024 8,062 1 View
I have data for milk fat 4.5 %, SD is 0.39, sample size is 10 Total milk production is 33 kg, Reviewer suggest me to add Total Fat Synthesis, If I calculate the total fat synthesis (TFS) in...
03 June 2024 9,894 3 View
I am trying to use a method from a paper by Do et al (https://www.sciencedirect.com/science/article/abs/pii/S0021967320311043?via%3Dihub) for a plant fingerprint. Short version: what could be...
01 June 2024 4,005 0 View
Many protocols for obtaining acute brain slices for electrophysiology involve cutting the brain in an ice-cold solution, then transferring the brain slices into a solution pre-warmed to...
31 May 2024 2,087 2 View
See Figure 1: Figure 1: Neocortical-cerebellar loops per language. A language command to speak is issued from the neocortex (cortex) which has access to the cerebellar cortex at the Purkinje...
30 May 2024 5,366 4 View
I am trying to perform eigenvalue analysis on two area system by Prabha Kundur On Powerfactory but for some reason can’t really get the values to be same as in the book, if anyone of you is...
29 May 2024 2,753 0 View
I've recently started to patch dentate gyrus granule cells in coronal mouse brain slices (300 μm). I'm using mixed NaCl/sucrose ACSF for cutting (ice-cold) and recovery (30 min at 35-37°C) and...
28 May 2024 3,711 1 View
I have several patients over 30 years with diagnosis of keratoconus based on single topography. As per guidelines only progressive disease should be treated by CXL. Should I do repeat topography...
27 May 2024 8,619 2 View
I'm doing research in veterinary science and topic is associated with ticks prevalence and seasonal variation in cow..I have randomly selected 560 cows as sample size and studing for prevalence...
26 May 2024 3,853 5 View
Understanding and Managing Constipation: A Comprehensive Rev... Can you explain the treatment options for adult constipation, including both non-pharmacological and pharmacological approaches?
25 May 2024 8,288 3 View
I'm working on cancer model in mice. In my free-drug treatment group, there is slow growth of tumor as compared to control (Saline) group. But in the nano-particle treated Group, the tumor...
25 May 2024 5,858 1 View