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Questions related to Mice
I'm using the Miltenyi Biotec NK MACS medium however the protocol says to use 5% AB Serum, is it possible to substitute the HS for HSA? IL-2 and IL-15 will be added to the media to help with NK...
23 March 2022 6,174 3 View
Hi all. I'm planning to incubate my primary hippocampal neuron culture in microglia-conditioned media, in which the microglia were stimulated with LPS+ATP to produce cytokines. As we use...
23 March 2022 4,872 0 View
Greetings, I have modeled a faulty system (Bowed rotor) in MATLAB. By increasing the sampling frequency, a frequency component appeared in the FFT graph (after the 1X), but the location (frequency...
23 March 2022 1,436 1 View
Hi researchers, I was wondering if anyone knows a way I could Isolate RNA and Protein from the same cell pellet. I'm not looking for a kit that allows me to do both at the same time unless that...
23 March 2022 8,964 7 View
I have been trying a protocol for T cell activation and differentiation using murine splenocytes in vitro, but I have been getting a lot of dead cells as indicated by flow cytometry. I am coating...
22 March 2022 5,154 2 View
Hi all, I am trying to grow human bone-marrow derived mesenchimal stem cells and they take ages to grow in a T75. When i get confluence after 2-3 weeks and passage them into a T175, they grow...
22 March 2022 1,577 5 View
I've been trying to cultivate BMDM into macrophages since a weak, and I'm using DMEM with 10% FBS and 5% pen strep. I changed the media three or four times and rinsed it with 1X PBS once. Although...
21 March 2022 5,106 3 View
I am having an exposure time problem with one of my colors. 647 Alexaluor My other colors expose for less than 1 second or at the most 3 seconds. the 647 can take 10+ to even 20 seconds. what...
20 March 2022 3,280 3 View
Hello, I am going to treat the mouse heart with a drug. I know that there is a method that typically uses the langendorff system to treat the heart. Without using the langendorff system, does...
17 March 2022 3,902 1 View
Dear all, Which is the best mounting medium for liver frozen sections stained with oil red O? An aqueous one as Ab104135 could be the solution (even if suggested for IF)? Thanks in advance.
17 March 2022 2,524 3 View
I am trying to isolate neutrophils from mouse bone marrow with percoll (62%). The goal is to stimualte the isolated neutrophils with LPS and afterwards lyse the cells for RNA analysis. I wonder...
16 March 2022 7,567 2 View
I have reacted a known glucose concentration (0.75mg/ml) with 1% w/v DNSA at a mole ratio of 1:1.05 and 1:2.1 mole ratio . The 1:2.1 ratio doesn't completely react i.e. I was expecting to obtain...
14 March 2022 2,646 0 View
I am trying to culture macrophages from the bone marrow of mice. I always worked with macrophages and had no problem culturing them but recently it has been hard to get them as most of them do not...
13 March 2022 7,622 3 View
We are trying to harvest and culture mouse primary nodose/jugular neurons. My isolation technique is good as I learned from Bradley Undem's lab. However, when I plate the cell suspension I get...
12 March 2022 3,903 2 View
How do I know if I am injecting into the tail vein of a mouse correctly? what are some tips for a successful injection.
11 March 2022 1,895 3 View
I have bones of mice that are PFA fixated, cryoembedded and cut into slices with the Kawamoto method. Now I am trying to stain intracellular markers on these sections. I have tried different...
11 March 2022 6,646 2 View
I am working on primary hippocampus neurons from mice. I am trying to look at the effect of amyloid beta on the neurons. I am looking at parameters like cell death, number of neurons in a field of...
10 March 2022 6,036 5 View
Hi all, I've been trying to troubleshoot using the In Situ Cell Death detection kit from Sigma/Merck with fluorescein for use on PFA-fixed rat brain sections. I'm currently trying the staining on...
10 March 2022 642 4 View
Hi everyone, I am working with P7 mouse hippocampal slices, The media looks clear after 10 days now but I can see small black dots in the slices. They don't show any mobility ( as expected in...
09 March 2022 1,242 2 View
Hi, it is my first time to do cryosections and immunostaining for mouse pancrea, and face a lot of technical issues. I will use GFP to stain the neural cells in pancreas, and have no idea how to...
07 March 2022 2,614 5 View
What is the current opinion about the Abercrombie-correction and its modifications? Most literature I find on the topic is at least 20 years old.
07 March 2022 1,308 1 View
All the steps were performed according to the SOP but the Internal control graph of any samples, as well as Positive control, was missing whereas the target genes are detected.
06 March 2022 5,597 2 View
Hi, I need to transfect mice lung fibroblasts with lenti-cre. I bought one from a company but it didn't work at all, efficiency's super low. Can anyone recommend the premade lentil-cre particles...
04 March 2022 3,520 2 View
Hey all, I'm working on synthesizing Gold nanoparticles (GNP) - antibodies conjugates for biomedical purposes. We are struggling to find an efficient method to sperate the unconjugated antibodies...
04 March 2022 8,958 4 View