Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,071 2 View
Thank you in advance for your support. We conducted a stratified random survey in 13 strata and obtained a sample size for each stratum. At the time of the survey we had a variable...
01 July 2024 280 3 View
A physical mix was in vitro evaluated on a torque rheometer. The composition was predominantly recycled polypropylene (PP), yet the melt flow index (MFI) was found to be excessively high....
25 June 2024 10,154 0 View
I know the neubauer method, but I hear about the recount of growth with spectrophotometer.
23 June 2024 9,295 1 View
I want to do my post doc outside Mexico can you recommend me where to apply?
19 May 2024 9,052 0 View
The research "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory" is a study that aims to assess the viability of replacing serum samples with...
09 April 2024 703 1 View
That is, how many milliliters are used in each surgery? I have searched for information regarding consumption by respiratory volume or per kg of weight but I cannot find information.
24 March 2024 6,744 1 View
Is there a direct and simple relationship between the solubility of a salt and the dielectric constant of the solvent or solvent mixture in which the salt is to be solubilised? For example: the...
21 March 2024 2,313 2 View
Me encuentro realizando una investigación sobre La Historia del Comercio Internacional de Colombia, En el Primer capítulo me encuentro abordando los imperios Latinoamericanos precoloniales como el...
21 March 2024 4,146 2 View
I'm researching about videogames and their effect on metacognition, actually I intend to develop a game with that specific intention. I'm tinkering with a puzzle game that gives feedback and maybe...
18 March 2024 1,145 2 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 647 0 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
05 August 2024 5,829 1 View
While living in Boston, which has a fine metro system, there were times that I would exit from underground at the wrong location, thereby expecting a particular visual scene only to be momentarily...
17 July 2024 3,237 10 View
Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...
01 July 2024 8,070 0 View
Has anyone tried co-culturing and SH-SY5Y cells with human astrocytes and differentiating ? I am planning to seed astrocytes, let them grow for 3 days and introduce SH-SY5Y cells into it. Ideally,...
01 July 2024 241 2 View
I am attempting to determine which current inputs (in uA) are subthreshold for inducing a burst of activity. This is because I am attempting to model DBS form epilepsy in vitro, which clinically...
23 June 2024 4,083 1 View
Receiving reviews of a paper (Tehovnik, Carvey et al. 2003) in which we electrically stimulated the visual cortex of primates to evoke saccadic eye movements under various behavioral conditions to...
19 June 2024 4,676 0 View
I used SD rats (E19) for cortical neuron extraction. Due to the need for subsequent special experiments, I cultured the neurons in a Corning PDL 384-well plate. The culture medium is Neurobasal +...
12 June 2024 1,714 4 View
I'm studying hippocampal pyramidal and granule cells in coronal mouse brain slices using patch-clamp. I use mixed NaCl/sucrose ACSF for cutting (ice-cold) and recovery (30 min at 35-37 °C), 0.5...
30 May 2024 3,472 1 View
Many protocols for obtaining acute brain slices for electrophysiology involve cutting the brain in an ice-cold solution, then transferring the brain slices into a solution pre-warmed to...
30 May 2024 2,055 2 View