Does long-term culture of murine Monocytes result in macrophage differentiation?

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How to analyze my MTT results?

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How to analyze ELISA results?

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OD value not different after dilution?

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How to analyze my MTT results?

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Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

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Does Napabucasin soluble in dichloromethane and in ethanol?

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Help request in CST microwave?

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Which cell line should we use to perform biological dosimetry experiments?

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Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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What could be some reasons why unstimulated monocytes are getting activated?

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Shiro Kanegasaki

Human peripheral monocytes cultured with GM-CSF and IL-4 differentiated to dendritic cells (DCs) and with GM-CSF alone to macrophages.

Article Suppression of Superoxide-Generating Ability during Differen...

Janina Moros

Hi Merel,

I only have experience with human monocytes. I would not recommend to culture them over a longer period. Adding a culturing step will change the biological characteristics of monocytes very rapidly.