Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
I'm trying to stain mouse blastocysts with antibodies for Cdx2 and Oct4, I've had great success with my Cdx2 antibody however the Oct4 staining is always hazy and non-specific. I'm using an...
20 September 2016 8,757 3 View
Hi I am able to do primary and subculture of human keratinocyte in monolayer . I want to stratify them in culture to make cell sheets. I have read about the use of high calcium and serum in the...
16 September 2016 6,197 3 View
Has anyone experiences in staining against iNOS in PFA-fixed and cryopreserved brain tissue of nude mice? I´d be very grateful for any suggestion: Protocol, company, concentration, pre-treatment,...
15 September 2016 4,338 5 View
I would like to detect motor neurons in mouse spinal cord as an alternative to violet crystal.
05 September 2016 3,036 7 View
How long can I keep the bones in NBF before processing for histology and/or immunohistochemistry?
30 August 2016 9,746 2 View
Stereology methods require tissue to be cut in thick sections (such as 50 or 60um in thickness), but it becomes a challenge for immunohistochemistry, as thick sections can be hard for antibodies...
26 August 2016 6,854 2 View
Hi, I'm working on an economic analysis looking at clinical outcomes for uterine fibroid treatment. I was wondering if you could apply different cycle times to the same model, as I would like to...
18 August 2016 3,307 6 View
an appropriate fixative medium for embryonic day 18.5 mouse head samples that will be used for immunohistochemistry . how long they should be fixed? Any link I should read for good sample...
16 August 2016 9,140 3 View
Ok: I have pictures of heart tissue with a staining of WGA, SMA and Dapi. I am looking for arteries. Now I am trying to measure the lumen of this arteries. But to avoid drawing a area by hand for...
11 August 2016 830 5 View
Now, I want to make paraffin wax with frozen tissue(Liver tissue). I check some information, it says that it is not suitable for frozen tissue to make paraffin wax, because tissue will be damaged...
05 August 2016 9,748 2 View
I am doing the c-Fos IHC on human postmortem brain tissue. The antibody I used is c-Fos (4): sc-52 from Santa Cruz which worked fantastic on mouse brain tissue. However, when it comes to the human...
04 August 2016 688 2 View
Hi all, I sectioned fresh frozen brains (CO2 euthanized rats) using a cryostat. I typically do immunohistochemistry with perfused free-floating brains, but I want to try it with fresh frozen...
03 August 2016 9,202 13 View
Hi everyone! I'm searching detailed protocol for teasing-fiber technique for immunohistochemistry. My approach to these technique is self made, with few indication and i need some help, in...
01 August 2016 9,358 1 View
I am trying to find the best protocol for free-floating frozen IR IHC.
28 July 2016 8,798 2 View
I am planning to do IHC with mice brain samples.will it be a good idea if i use paraffin fixed samples for the same and then do a confocal microscopy? It will be a great help if someone help me...
27 July 2016 9,119 4 View
hello every body!I am transferring proteins on a 45 um NC membrane. when working with 90 volts for 100 mins, complete transfer in not achieved, as bands can be seen on the gel when stained with...
27 July 2016 8,173 8 View
1- I read sometimes people added blocking solution after primary Ab and after that added secondary Ab, while, other people normally do before primary Ab and prior that added secondary Ab. - My...
17 July 2016 945 4 View
I'm currently investigating the effects of a drug in attenuating brain injury following hypoxic-ischaemic (HI) insult. Does anyone have any advice about what would be the best histochemical marker...
13 July 2016 1,591 5 View
We've tried a few protocols on our autostainer, but haven't had got anything working yet. I have heard rumors of people getting it to stain rat tissue . I specifically want to stain vascular...
05 July 2016 3,408 3 View
I need to make immunohistochemistry on rat tissue (spleen and skin). The tissues were fixed 24 hours in formol and 4 weeks in ethanol. I can use cytokeratin and CD68 on these tissues but I am...
30 June 2016 3,143 3 View
Hi, doing retina immunohistochemistry and I have done PKC-alpha and glutamine synthetase (GS) staining. Was wondering if I could get new information out of anti-bassoon staining? or PKC-alpha and...
22 June 2016 537 3 View
For my stainings of 3 um thick slides of mouse liver tissue (fixed for 24 hrs at 4C, 4% paraformaldehyde) I accidentally didn´t use Triton X-100 to permeabilize cell membranes. I did it the same...
19 June 2016 9,934 9 View
hi, i just harvested xenograft from naked mice after injected human tumor cell for tumor proliferation assay in vivo. i want to detect KI-67 expression in the tumorsection by IHC assay. but i am...
17 June 2016 8,125 1 View
Greetings, An established in-house protocol has so far proven unsuccessful to stain tumor samples for macrophages using F4/80 (CI:A3-1) : sc-59171. It would be highly appreciated if someone can...
15 June 2016 9,524 2 View