Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
I am doing the c-Fos IHC on human postmortem brain tissue. The antibody I used is c-Fos (4): sc-52 from Santa Cruz which worked fantastic on mouse brain tissue. However, when it comes to the human...
04 August 2016 599 2 View
Hi all, I sectioned fresh frozen brains (CO2 euthanized rats) using a cryostat. I typically do immunohistochemistry with perfused free-floating brains, but I want to try it with fresh frozen...
03 August 2016 9,092 13 View
Hi everyone! I'm searching detailed protocol for teasing-fiber technique for immunohistochemistry. My approach to these technique is self made, with few indication and i need some help, in...
01 August 2016 9,268 1 View
I am trying to find the best protocol for free-floating frozen IR IHC.
28 July 2016 8,681 2 View
I am planning to do IHC with mice brain samples.will it be a good idea if i use paraffin fixed samples for the same and then do a confocal microscopy? It will be a great help if someone help me...
27 July 2016 9,011 4 View
hello every body!I am transferring proteins on a 45 um NC membrane. when working with 90 volts for 100 mins, complete transfer in not achieved, as bands can be seen on the gel when stained with...
27 July 2016 8,065 8 View
1- I read sometimes people added blocking solution after primary Ab and after that added secondary Ab, while, other people normally do before primary Ab and prior that added secondary Ab. - My...
17 July 2016 865 4 View
I'm currently investigating the effects of a drug in attenuating brain injury following hypoxic-ischaemic (HI) insult. Does anyone have any advice about what would be the best histochemical marker...
13 July 2016 1,486 5 View
We've tried a few protocols on our autostainer, but haven't had got anything working yet. I have heard rumors of people getting it to stain rat tissue . I specifically want to stain vascular...
05 July 2016 3,332 3 View
I need to make immunohistochemistry on rat tissue (spleen and skin). The tissues were fixed 24 hours in formol and 4 weeks in ethanol. I can use cytokeratin and CD68 on these tissues but I am...
30 June 2016 3,035 3 View
Hi, doing retina immunohistochemistry and I have done PKC-alpha and glutamine synthetase (GS) staining. Was wondering if I could get new information out of anti-bassoon staining? or PKC-alpha and...
22 June 2016 486 3 View
For my stainings of 3 um thick slides of mouse liver tissue (fixed for 24 hrs at 4C, 4% paraformaldehyde) I accidentally didn´t use Triton X-100 to permeabilize cell membranes. I did it the same...
19 June 2016 9,856 9 View
hi, i just harvested xenograft from naked mice after injected human tumor cell for tumor proliferation assay in vivo. i want to detect KI-67 expression in the tumorsection by IHC assay. but i am...
17 June 2016 8,054 1 View
Greetings, An established in-house protocol has so far proven unsuccessful to stain tumor samples for macrophages using F4/80 (CI:A3-1) : sc-59171. It would be highly appreciated if someone can...
15 June 2016 9,419 2 View
I am trying to run IL22 for IHC and I do not know which one should I buy as a friend of mine is struggling to get a positive results from IL22 (ab 18499)
12 June 2016 3,742 1 View
Hi Everyone, May i ask who has normalized his/her IP results for interaction? especially considering equal over-expression, equal loading into a gel and equal pull-down across samples? Thanks,
08 June 2016 5,492 3 View
I had a confusion with one immunohistochemistry slide from a patient with Non-Segmental Vitiligo. I stained The slide against CD3+ marker, and there were some folding in the tissue but the rest of...
25 May 2016 6,466 1 View
Hi I am try to show that an organotypic skin model I have built closely resembles the structure of human skin by performing immunofluorescence with antibodies directed against a number of antigens...
23 May 2016 5,377 2 View
Hi, I am wondering whether it's possible to detect (by IHC and/or IF) phosphorylation of SMADs (SMAD 1/5/8) in isolated murine cells (alveolar, peritoneal or bone marrow macrophages). Please point...
18 May 2016 3,583 1 View
Looking for advice/suggestions/critique on a protocol for a rapid stain (
17 May 2016 2,359 3 View
I work with microglial cells of difficult adhesion, can't that adhere to any support. so far deal with a coating of gelatin, collagen, poly-d-lysine. Anyone have any other idea of some strong...
04 May 2016 5,097 3 View
Anyone knows if the procedure of deparaffinization can alter the structure/composition of plasma membranes?
28 April 2016 9,467 6 View
I understand that autofluorescence is particularly problematic with placental and fetal membrane tissue - are there ways around this, or should I stick with peroxidase secondary antibodies?
27 April 2016 2,912 4 View
Using immunohistochemisty, I want to determine if a postsynaptic protein is juxtaposed to C-fibers in lamina II of the spinal cord superficial dorsal horn. There are plenty of marker proteins for...
26 April 2016 9,190 1 View