Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
Has anyone experiences in staining against iNOS in PFA-fixed and cryopreserved brain tissue of nude mice? I´d be very grateful for any suggestion: Protocol, company, concentration, pre-treatment,...
15 September 2016 4,279 5 View
I would like to detect motor neurons in mouse spinal cord as an alternative to violet crystal.
05 September 2016 2,952 7 View
How long can I keep the bones in NBF before processing for histology and/or immunohistochemistry?
30 August 2016 9,675 2 View
Stereology methods require tissue to be cut in thick sections (such as 50 or 60um in thickness), but it becomes a challenge for immunohistochemistry, as thick sections can be hard for antibodies...
26 August 2016 6,748 2 View
Hi, I'm working on an economic analysis looking at clinical outcomes for uterine fibroid treatment. I was wondering if you could apply different cycle times to the same model, as I would like to...
18 August 2016 3,226 6 View
an appropriate fixative medium for embryonic day 18.5 mouse head samples that will be used for immunohistochemistry . how long they should be fixed? Any link I should read for good sample...
16 August 2016 9,025 3 View
Ok: I have pictures of heart tissue with a staining of WGA, SMA and Dapi. I am looking for arteries. Now I am trying to measure the lumen of this arteries. But to avoid drawing a area by hand for...
11 August 2016 748 5 View
Now, I want to make paraffin wax with frozen tissue(Liver tissue). I check some information, it says that it is not suitable for frozen tissue to make paraffin wax, because tissue will be damaged...
05 August 2016 9,678 2 View
I am doing the c-Fos IHC on human postmortem brain tissue. The antibody I used is c-Fos (4): sc-52 from Santa Cruz which worked fantastic on mouse brain tissue. However, when it comes to the human...
04 August 2016 606 2 View
Hi all, I sectioned fresh frozen brains (CO2 euthanized rats) using a cryostat. I typically do immunohistochemistry with perfused free-floating brains, but I want to try it with fresh frozen...
03 August 2016 9,098 13 View
Hi everyone! I'm searching detailed protocol for teasing-fiber technique for immunohistochemistry. My approach to these technique is self made, with few indication and i need some help, in...
01 August 2016 9,275 1 View
I am trying to find the best protocol for free-floating frozen IR IHC.
28 July 2016 8,692 2 View
I am planning to do IHC with mice brain samples.will it be a good idea if i use paraffin fixed samples for the same and then do a confocal microscopy? It will be a great help if someone help me...
27 July 2016 9,019 4 View
hello every body!I am transferring proteins on a 45 um NC membrane. when working with 90 volts for 100 mins, complete transfer in not achieved, as bands can be seen on the gel when stained with...
27 July 2016 8,073 8 View
1- I read sometimes people added blocking solution after primary Ab and after that added secondary Ab, while, other people normally do before primary Ab and prior that added secondary Ab. - My...
17 July 2016 871 4 View
I'm currently investigating the effects of a drug in attenuating brain injury following hypoxic-ischaemic (HI) insult. Does anyone have any advice about what would be the best histochemical marker...
13 July 2016 1,495 5 View
We've tried a few protocols on our autostainer, but haven't had got anything working yet. I have heard rumors of people getting it to stain rat tissue . I specifically want to stain vascular...
05 July 2016 3,338 3 View
I need to make immunohistochemistry on rat tissue (spleen and skin). The tissues were fixed 24 hours in formol and 4 weeks in ethanol. I can use cytokeratin and CD68 on these tissues but I am...
30 June 2016 3,050 3 View
Hi, doing retina immunohistochemistry and I have done PKC-alpha and glutamine synthetase (GS) staining. Was wondering if I could get new information out of anti-bassoon staining? or PKC-alpha and...
22 June 2016 490 3 View
For my stainings of 3 um thick slides of mouse liver tissue (fixed for 24 hrs at 4C, 4% paraformaldehyde) I accidentally didn´t use Triton X-100 to permeabilize cell membranes. I did it the same...
19 June 2016 9,868 9 View
hi, i just harvested xenograft from naked mice after injected human tumor cell for tumor proliferation assay in vivo. i want to detect KI-67 expression in the tumorsection by IHC assay. but i am...
17 June 2016 8,063 1 View
Greetings, An established in-house protocol has so far proven unsuccessful to stain tumor samples for macrophages using F4/80 (CI:A3-1) : sc-59171. It would be highly appreciated if someone can...
15 June 2016 9,437 2 View
I am trying to run IL22 for IHC and I do not know which one should I buy as a friend of mine is struggling to get a positive results from IL22 (ab 18499)
12 June 2016 3,746 1 View
Hi Everyone, May i ask who has normalized his/her IP results for interaction? especially considering equal over-expression, equal loading into a gel and equal pull-down across samples? Thanks,
08 June 2016 5,512 3 View