Histocytochemistry

Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake,... | Contact experts in Histocytochemistry to get answers

561 views 11 posts

Questions related to Histocytochemistry

Are there differences of expression levels of tumor markers in the same patient?

Hello, I'm wondering if there are there differences of expression levels of tumor markers in the same patient. For example, in a same patient who has diffuse large B-cell lymphoma, would the...

01 January 2016 1,838 5 View

Does anyone have a clue for a nice TMA DAB staining?

I have the following problem: I'm doing a usual DAB staining (1°Ab, 2° Ab conjugated, HRP and Tyramide and then DAB for chromogen detection) that works well. My issue is that many of the TMA...

09 September 2015 5,738 4 View

What is the most suitable histology stain to visualize fibrin in tissue sections?

I want to detect fibrin in harvested organs. Masson's trichome, Martius Scarlet Blue and Carstairs all seem to be used for fibrin staining. Anybody knows which one would work the best ? Or should...

03 March 2015 9,820 5 View

Which would be a better counterstain for NiDAB IHC: cresyl violet or neutral red?

Other histological stains (e.g., methyl green) aside, which would be a better counterstain for nickel-enhanced DAB immunohsitochemistry: cresyl violet or neutral red? Should I alter my staining...

01 January 2015 1,013 3 View

Can anyone recommend a protocol to stain the vasculature?

Young, migrating neurons are often seen riding or in close proximity to the vasculature. I am looking for a protocol to stain the vasculature that will not interfere with DCX/BrdU and cytoplasmic...

11 November 2014 3,479 7 View

Does anybody know how to convert mouse IL-28B/INF-l3 from Ug to working units?

I have to stimulate my cells with IFN lambda or IFN beta and compare the levels of stimulation. I need to stimulate my cells at the same concentration expressed in untis/ml. Also because the...

10 October 2014 1,882 1 View

How do I prepare microalgae cells for flow cytometry ?

I want to staning DNA whit DAPI ... lysis cell wall whitout digesting the Nuclear membrane

26 September 2014 9,203 1 View

How do I prepare microalgae cells for flow cytometry ?

I want to staning DNA whit DAPI ... lysis cell wall whitout digesting the Nuclear membrane

09 September 2014 2,378 1 View

Badges
Science topic