Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
1,179 views 669 posts
Questions related to Immunohistochemistry
I had a confusion with one immunohistochemistry slide from a patient with Non-Segmental Vitiligo. I stained The slide against CD3+ marker, and there were some folding in the tissue but the rest of...
25 May 2016 6,477 1 View
Hi I am try to show that an organotypic skin model I have built closely resembles the structure of human skin by performing immunofluorescence with antibodies directed against a number of antigens...
23 May 2016 5,386 2 View
Hi, I am wondering whether it's possible to detect (by IHC and/or IF) phosphorylation of SMADs (SMAD 1/5/8) in isolated murine cells (alveolar, peritoneal or bone marrow macrophages). Please point...
18 May 2016 3,590 1 View
Looking for advice/suggestions/critique on a protocol for a rapid stain (
17 May 2016 2,367 3 View
I work with microglial cells of difficult adhesion, can't that adhere to any support. so far deal with a coating of gelatin, collagen, poly-d-lysine. Anyone have any other idea of some strong...
04 May 2016 5,109 3 View
Anyone knows if the procedure of deparaffinization can alter the structure/composition of plasma membranes?
28 April 2016 9,480 6 View
I understand that autofluorescence is particularly problematic with placental and fetal membrane tissue - are there ways around this, or should I stick with peroxidase secondary antibodies?
27 April 2016 2,922 4 View
Using immunohistochemisty, I want to determine if a postsynaptic protein is juxtaposed to C-fibers in lamina II of the spinal cord superficial dorsal horn. There are plenty of marker proteins for...
26 April 2016 9,205 1 View
I'm going to quantify proteoglycans in spine. I use Imagej Software. I open the file then define the threshold and choose dark background. Then I choose 5 ROIs and measure integrated density for...
23 April 2016 3,197 3 View
The experiment involves uptake of a fluorescent compound and confocal imaging. Attempts to fix the cells with 4% PFA and staining 1:50 with Alexa Fluor 633-conjugated phalloidin in accordance with...
23 April 2016 257 3 View
I need to demonstrate the presence of Fusobacterium in polymicrobial infection using immuno-histochemistry
21 April 2016 6,609 1 View
Using a glioblastoma cell line. Fluorescent microscopy
20 April 2016 7,442 3 View
recently, I stained BDNF of mouse brain by immunohistochemistry. the resluts showed that the protein mainly express in ependyma. is the results right?
15 April 2016 6,033 3 View
I need to perform immunohistochemistry on rat free-floating brain sections. Rats were perfused with 4% paraformaldehyde. I have tried a couple antibodies but have not seen consistent results. If...
12 April 2016 7,949 7 View
After 21 days of differentiation the pellet will be harvested for histology & immunohistochemistry, What is the best way to harvest the pellet without affecting the cell morphology? Please...
11 April 2016 2,842 4 View
I found this paper using Citraconic Anhydride and Heat (please see attached) and I would like to know if anyone has successfully replicated it.
05 April 2016 2,353 5 View
IHC-P Tissue IF application
01 April 2016 538 3 View
I am doing immunohistochemical staining with DAB development on rat brain tissue. The first immunostainings with rabbit anti-alpha-synuclein and rabbit anti-GFP antibodies went well and look as...
11 March 2016 3,980 9 View
I tried CD11b antibody immunohistochemistry on frozen-section 4% PFA fixed tissues (post-fixed for 48hrs) using the following protocol: 1% H2O2 in PBS and washed. Preincubution in 5% Normal Horse...
09 March 2016 9,441 4 View
Immunohistochemistry
10 January 2016 3,751 1 View
Is there a way to rescue my tissue sections after they have dried out during primary antibody incubation overnight? If I boil the slides in antigen retrieval solution and repeat the staining...
11 November 2015 4,116 5 View
We are looking for an antibody that can detect murine VHL (von Hippel-Lindau) via immunohistochemistry, preferably on FFPE sections. Many thanks and best wishes! Thorsten
04 September 2015 9,809 3 View
I am working in an in vivo electrophysiology lab, and we do not have the resources to do our own immunohistochemistry. Initially, we went to the university's pathology core to develop a protocol...
08 April 2015 5,295 6 View
Hello,all. I will perform immunohistochemical staining on brain slices to separate different regions of mouse brain . As we all know, AchE satining can help us recognize striatum,I just wondered...
07 April 2015 2,262 2 View