Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
Currently I am doing research into muscle composition in mice. The focus is on two muscles with a diameter of around a millimeter, which I am sectioning with a Cryostat. The muscles were on...
15 November 2016 6,110 2 View
I know tat ImageJ has options to count cells either manually or automatically but my protein of interest lights up the synapses and hence can't use both. I thought of measuring the mean grayscale...
05 November 2016 4,759 2 View
I am looking for GCPRs in heart. I performed IHC and now want to quantify and report it. I am totally confused with quantification methods completely. I found some articles but they talked about...
03 November 2016 5,545 6 View
We want to carry out IHC in paraffin embedded liver tissue for hypoxic damage and oxidative stress. There seems to be so many different types of markers to look at. Does anyone have experience in...
02 November 2016 821 3 View
I have tissue that has been frozen in N2 without supporting medium (OCT). Could I put the tissue in OCT and put next in isopentane just to solidify the block and make the sectioning possible.
02 November 2016 5,987 7 View
I do have a knock-in mice with a 3xFLAG tagged protein. I want to check its expression with immunohistochemistry in paraffin sections. Has anyone tried? Would it be better in cryostat sections?
28 October 2016 4,017 1 View
We have observed a massive transcriptional regulation of FOLH1 in cell cultures. This protein is also known as Prostate-Specific Membrane Antigen or Glutamate Carboxypeptidase II. However we were...
26 October 2016 4,746 2 View
We have some rats being sacrificed for access to their brains for immunohistochemsitry. But since they are already being sacrificed, I was wondering how to go about removing their eyes as well for...
22 October 2016 9,162 2 View
Can someone help me to find an easy way to calculate the area affected with A-beta plaques in images of DAB stained section brain sections.
22 October 2016 9,409 2 View
Aquaporins are transmembrane proteins.
10 October 2016 8,412 1 View
Hi all! Can anyone tell me the best way to identify infiltrating immune cells by IHC both in mouse and human tissue samples (normal liver/liver cancer, paraffin-embedded)? I.e., the best marker(s)...
03 October 2016 388 2 View
Hi, We have generated trangenic mice overexpressing Myc-tagged transgene. I can detect the transgene using c-Myc (9E10) or Myc-tag antibody (Cell Signaling) by immunohistochemistry. Is there a...
29 September 2016 8,560 1 View
Immunohistochemistry Immunocytocheistry
24 September 2016 741 5 View
I'm recently working on the Gomori's trichrome staining on adult mouse limb. The whole lower limbs (skinned) were fixed in 4% PFA at 4C , decalcified in EDTA, embeded in OCT and cut as 20um...
23 September 2016 8,546 5 View
I'm trying to stain mouse blastocysts with antibodies for Cdx2 and Oct4, I've had great success with my Cdx2 antibody however the Oct4 staining is always hazy and non-specific. I'm using an...
20 September 2016 8,677 3 View
Hi I am able to do primary and subculture of human keratinocyte in monolayer . I want to stratify them in culture to make cell sheets. I have read about the use of high calcium and serum in the...
16 September 2016 6,127 3 View
Has anyone experiences in staining against iNOS in PFA-fixed and cryopreserved brain tissue of nude mice? I´d be very grateful for any suggestion: Protocol, company, concentration, pre-treatment,...
15 September 2016 4,273 5 View
I would like to detect motor neurons in mouse spinal cord as an alternative to violet crystal.
05 September 2016 2,939 7 View
How long can I keep the bones in NBF before processing for histology and/or immunohistochemistry?
30 August 2016 9,666 2 View
Stereology methods require tissue to be cut in thick sections (such as 50 or 60um in thickness), but it becomes a challenge for immunohistochemistry, as thick sections can be hard for antibodies...
26 August 2016 6,728 2 View
Hi, I'm working on an economic analysis looking at clinical outcomes for uterine fibroid treatment. I was wondering if you could apply different cycle times to the same model, as I would like to...
18 August 2016 3,215 6 View
an appropriate fixative medium for embryonic day 18.5 mouse head samples that will be used for immunohistochemistry . how long they should be fixed? Any link I should read for good sample...
16 August 2016 9,010 3 View
Ok: I have pictures of heart tissue with a staining of WGA, SMA and Dapi. I am looking for arteries. Now I am trying to measure the lumen of this arteries. But to avoid drawing a area by hand for...
11 August 2016 736 5 View
Now, I want to make paraffin wax with frozen tissue(Liver tissue). I check some information, it says that it is not suitable for frozen tissue to make paraffin wax, because tissue will be damaged...
05 August 2016 9,674 2 View