Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
I am performing IHC using mouse brain slices. I am performing double labeling and the blocking buffer I am using is normal goat serum. One target remains constant in my experiments, and the second...
05 January 2017 5,091 3 View
Hello everyone, In order to cut 10 µm slices of mammospheres with cryostat, I had to colour them with eosin to be able to follow them while cutting.What a bad idea you'll tell me, because eosin...
20 December 2016 5,425 5 View
My antibody didn't work at all. I have mouse brains, 30 um cryosectioned, and tried different concentrations of tween, as well as both formamide and SSC treatment to no avail.
19 December 2016 6,975 3 View
In my lab we generally used to do immunocytochemistry regularly but now its not working that means it does not show any signals after secondary antibody addition with background or almost no stain...
18 December 2016 5,130 7 View
my cells contains luciferase, when i injected cells into mice, the Bioluminescence imaging showed well. but when i do the tissue immunohistochemistry, i found the luciferase express in nuclea,...
16 December 2016 2,385 3 View
Hi all! I am currently working on a project where we have gone from using mouse embryos (E7.5-9.0) to pig embryos (E.17-20). We have notices of late a lot of issues with the technical aspect of...
15 December 2016 2,855 4 View
Dear researchgaters, I have a question regarding staining protocols for oxidative tissue markers via IHC (4-HNE, MDA-2, 8-0HdG etc). I have found that these markers work in cryo stored material,...
13 December 2016 8,331 3 View
CD3, CD5 Parrafin section immunohistology. Dear Colleagues, I have also problem in Immunostaining of Parrafin sections of Rat sciatic nerve and spleen with CD3 and CD5. I am also using rabbit...
12 December 2016 5,340 3 View
Hello, I'm having some trouble with my negative control slides when I do immunofluorescence. I only work with frozen muscle tissue, and I'm trying to optimize a number of antibodies. For some of...
08 December 2016 3,604 7 View
Hi everyone, I plan to perfuse-fix my mice with 4% PFA through the heart, carefully extract the brain and then post-fix the brain overnight in 4% PFA for 12-16 hours. I plan to paraffin embed them...
17 November 2016 3,402 3 View
Currently I am doing research into muscle composition in mice. The focus is on two muscles with a diameter of around a millimeter, which I am sectioning with a Cryostat. The muscles were on...
15 November 2016 6,149 2 View
I know tat ImageJ has options to count cells either manually or automatically but my protein of interest lights up the synapses and hence can't use both. I thought of measuring the mean grayscale...
05 November 2016 4,871 2 View
I am looking for GCPRs in heart. I performed IHC and now want to quantify and report it. I am totally confused with quantification methods completely. I found some articles but they talked about...
03 November 2016 5,594 6 View
We want to carry out IHC in paraffin embedded liver tissue for hypoxic damage and oxidative stress. There seems to be so many different types of markers to look at. Does anyone have experience in...
02 November 2016 903 3 View
I have tissue that has been frozen in N2 without supporting medium (OCT). Could I put the tissue in OCT and put next in isopentane just to solidify the block and make the sectioning possible.
02 November 2016 6,047 7 View
I do have a knock-in mice with a 3xFLAG tagged protein. I want to check its expression with immunohistochemistry in paraffin sections. Has anyone tried? Would it be better in cryostat sections?
28 October 2016 4,100 1 View
We have observed a massive transcriptional regulation of FOLH1 in cell cultures. This protein is also known as Prostate-Specific Membrane Antigen or Glutamate Carboxypeptidase II. However we were...
26 October 2016 4,908 2 View
We have some rats being sacrificed for access to their brains for immunohistochemsitry. But since they are already being sacrificed, I was wondering how to go about removing their eyes as well for...
22 October 2016 9,217 2 View
Can someone help me to find an easy way to calculate the area affected with A-beta plaques in images of DAB stained section brain sections.
22 October 2016 9,453 2 View
Aquaporins are transmembrane proteins.
10 October 2016 8,474 1 View
Hi all! Can anyone tell me the best way to identify infiltrating immune cells by IHC both in mouse and human tissue samples (normal liver/liver cancer, paraffin-embedded)? I.e., the best marker(s)...
03 October 2016 489 2 View
Hi, We have generated trangenic mice overexpressing Myc-tagged transgene. I can detect the transgene using c-Myc (9E10) or Myc-tag antibody (Cell Signaling) by immunohistochemistry. Is there a...
29 September 2016 8,609 1 View
Immunohistochemistry Immunocytocheistry
24 September 2016 815 5 View
I'm recently working on the Gomori's trichrome staining on adult mouse limb. The whole lower limbs (skinned) were fixed in 4% PFA at 4C , decalcified in EDTA, embeded in OCT and cut as 20um...
23 September 2016 8,605 5 View