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Questions related to Histological Techniques
Dear fellow scientists, For our research in skin development we are collecting mouse embryos at different time points during embryogenesis. We now got an idea to do some further analysis for...
21 November 2022 2,976 0 View
I accidentally observed embryo induction by placing a sample of Anthurium leaves in callus induction medium without subculture after two months in the dark at 29 ° C.
20 October 2020 2,092 1 View
I am following this protocol (EB mediated version)Directed differentiation of forebrain GABA interneurons from... which gives me Gad1 positive neuron networks around day 45. I am trying to...
10 July 2020 4,871 0 View
I have done histological technique for pancrease and I need to know the special dyes to stain the endocrine cells in the pancreas
02 April 2020 6,261 8 View
We ard injecting an expensive chemical to pre frontal cortex of rat brain via sterotaxis guided by coordinates from brain atlas. We need to check if the injection was successful by a trial with...
27 February 2020 8,897 4 View
I'm currently trying to induce and identify mineralization in my SaOS-2 cells. The issue I'm having is that the whole well will stain red, and of course this intensity depends on how long the...
27 January 2020 6,591 5 View
I am currently trying to do FISH on human skin biopsies that have been routinely fixed in formalin and embedded in paraffin. I am using the CEN X/Yq12 probes from ZytoVision and their ZytoLight...
11 October 2019 6,105 4 View
Hello there, I want to snap-freeze mouse kidneys for immunofluorescence using isopentane surrounded by liquid nitrogen (1/4 of kidney for 1 min and then rapidly in -80°C freezer). Is there...
27 July 2019 5,201 2 View
Hello everyone! On my previous job I worked with Ventana Benchmark Ultra and performed various IHC stainigs on this device. As far as I can remember, engineer said, that after finishing of...
22 February 2019 3,583 2 View
Hello everybody, I have been working for several years on sea urchin gonad development using histological techniques, now I am going to start working on the histology of fish larvae (Pagrus pagrus...
27 April 2018 2,138 8 View
Dear all, I am looking for someone who worked with Sub X clearing agent and CLEARENE (made by Leica) and can tell my 1) which is better (less harmful to health and gives better histological...
29 January 2018 8,830 2 View
Eyes are fixed in Davidson's for 24hrs. The debris is most present in H&E stained slides.
24 January 2018 6,508 2 View
Hi, Does anybody have any experience with histologically processing arterial plaque tissue which may contain calcified regions? I understand decalcifying agents such as EDTA can be used however...
25 November 2017 7,390 7 View
I need to examine the digestive tract (start at mouth to anus) and try to do longitudinal sectioning of ponyfishes (size 3-4 cm). Should I dissect abdominal cavity before embedding or embedded the...
08 September 2017 4,320 1 View
I'm having some trouble trying to stain for endothelial cell in rat brain tissues. My positive control works for CD31, but my samples are not coming up. I'm wondering if it is my tissue...
26 July 2017 4,631 3 View
I took four slides out from -80 freezer. Then I realized one of the reagents was over but I couldn't see it before because the container is dark. What can I do? Do another staining or can I...
26 July 2017 8,106 3 View
We have mouse paws that were intended for other uses and stored in 70 or 100% ethanol for a year. What are my chances of being able to post-fix in formalin, then paraffin embed/section, to perform...
12 July 2017 747 3 View
I want to prepare fixation buffer 4% PFA/PBS but I'm confused with the methods I've come across. I tried to follow this one method whereby I dissolved 4g of PFA (merck, for synthesis) in 50 ml of...
10 July 2017 8,331 5 View
I want to cut 25μm longitude section of mouse gastrocnemius muscle in order to stain the NMJ with synaptophysin and alpha-Bungarotoxin conjugated with Alex 594. Here is the frozen section protocol...
08 July 2017 586 10 View
As the pic shows, the brown(such as black box) means the positive area of β-tubulin, and the white area is the negative area of β-tubulin. Tubulin is a kind of skeleton protein, in my opinion, if...
27 June 2017 9,905 1 View
Hello! I am going to do FISH and I have to make my experiment soon, but the probes are not here yet. How can I better store the cells and for how long? I saw in one protocol that you can leave in...
27 June 2017 7,703 4 View
I'm trying to do IHC in zebrafish paraffin tissues, and I saw this protocol.
20 June 2017 8,344 3 View
Hello, Our embedder is consistently overheating the wax to around 70C and we are unsure if this will damage the tissue for later IHC or make the block unsectionable. If you could provide any...
20 June 2017 1,879 4 View
We are trying to visualize cat dander on cat fur using light microscopy, We tried a various simple staining methods, but Parker's Black seems to be the best to date. Any ideas would be...
17 June 2017 8,041 4 View