What is the effect on the conductivity of the thin film during the process of thermal evaporation ?

Why thermally evaporated (VCM 600 V1 configuration of the thermal evaporator) CdTe film found n-type not p-type conductivity whereas all CdTe films deposited by varying the source to substrate...

23 November 2020 7,427 3 View

Can I analyse NGS DATA from an EXCEL spreadsheet?

Hi, I have Fold expression of RNAseq data obtained from Ion Proton sequencer and got the fold change data with mean in an excel sheet. now can I analyse this fold change data using any...

20 October 2020 2,240 3 View

Monolitic construction of brick masonary and concrete simultaniously have any effect ?

Concrete column casting after masonary completed monolithic.

17 August 2020 7,172 3 View

Rentraint Corner of building if need extra stitching diagonal reinforcement ?

If temperature reinforcement already provided ,at reentraint corner need extra diagonal reinforcement ,if needed then what amount of steel.

12 August 2020 1,707 2 View

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26 July 2020 1,511 0 View

How to calculate Time Average data (WSS, Velocity, Pressure) in ANSYS Fluent ?

I am doing a blood flow research for which Time average data of Velocity and Wall shear stress is important. The study is transient and my flow time is 0.5 sec. I save data with time step size of...

07 July 2020 4,323 2 View

What is the best management strategy for Diamond Back Moth (DBM) in cole crops?

What should be the best managemnt option for suppressing the DBM problem that farmer can take into consideration?

11 May 2020 4,280 1 View

Is it a good idea to retract a submitted article as the journal is taking too much time to make a decision?

I had submitted a research article related to the field of mechanical engineering (CFD) on the last December, 2019 in a Scopus Indexed journal. It has been around 6 months but the journal...

11 May 2020 9,286 8 View

Where can I get details of SARS-CoV-2 infected patients?

I have been working on peptide vaccine for SARS-CoV-2 or COVID-19 and also recently published some analysis on mutational changes in this virus. It would have been really helpful if I could get...

05 May 2020 2,749 9 View

How does the chemical etching (using HF) affect the temperature sensitivity of a fiber Bragg grating (FBG) inscribed in silica fiber?

Due to chemical etching, the cladding diameter of the silica fibre decreases with time. How does this reduced cladding diameter affect the temperature sensitivity of an FBG, which is inscribed on...

17 April 2020 4,771 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View