Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
I am new to the immunohistochemistry world and am currently about to teach myself the basics on some sections of C57BL/6 mouse lung. Does anyone have any good antibody recommendations or proteins...
29 October 2019 655 3 View
We are currently trying to phenotype the expression of a cell-adhesion molecule in Schwann cells and we would like to assess if there is differential expression of this molecule in myelinated and...
17 October 2019 3,726 2 View
I am developing an immunohistochemical protocol for an oxidative stress marker, but I am in doubt between the use of 1% BSA or 5% milk.
15 October 2019 6,078 2 View
My cells are fixed on a coverslip and are stained with DAPI, Cytokeratin and CD45. What steps should I take after staining the cells?
19 September 2019 1,523 4 View
I have stained some sections of mouse intestinal epithelium for phosphorylated ERK. I would like to count the number of DAB positive cells, and express that as a % of the total number of cells...
11 September 2019 199 8 View
I am trying to quantify my images for a poster using ImageJ, but am having a hard time because they aren't white balanced. Is there a free application I can download which will allow me to white...
31 July 2019 3,217 5 View
.. My Rat spinal cord samples are preserved in sucrose 30% .. I cut the sections using cryostat at 40 micron, All my buffer solutions for 1ry & 2ry Antibodies, blocking solution, washes are...
26 July 2019 2,820 8 View
Does anyone know of any good hypothalamic nuclei markers that can be used for immunohistochemistry purposes?
18 June 2019 4,095 3 View
The endometrial biopsy was taken in the mid-lutheal phase of the menstrual cycle from a woman with recurrent implantation failure (RIF). Extremely high percentage of stromal and luminal epithelial...
12 June 2019 2,709 1 View
I'm trying free-floating IHC instead of slide-mounted for mouse brain sections and am currently trying to figure out the best blocking media for my purpose. Beyond something like an appropriate...
30 May 2019 388 4 View
Has anybody ever tried to perform antigen retrieval on frozen tissue sections by incubation in sucrose? We tried that in case of murine iNOS in the spinal cord and it works pretty nice. However, I...
29 May 2019 746 3 View
Hi guys. I keep getting staining on void area of tissue (breast tumor tissues). As you can see both in my negative control(without primary antibody) and positive control, there are stretch...
17 May 2019 8,453 5 View
I am currently doing immunohistochemistry to look at a membrane antigen, on fixed embedded samples. I want to amplify the signal (low) I am getting, and was wondering which kind of amplification...
25 April 2019 5,336 5 View
Is there a recommendation for a software/FiJi-ImageJ plugin/workflow for quantifying ratio of expression of a protein between nucleus and cytoplasm (or the nuclei and the rest of the photo) in...
25 April 2019 8,144 2 View
Both rabbit (anti-Sox2) and mouse (anti-PCNA) primary antibodies work in single IHC but not in a double IHC. This is true for a simple mix of both antibodies as well as a sequential IHC. This also...
23 April 2019 6,683 8 View
Hello, I need to look for expression of IDO on myeloid dendritic cells (CD1c, CD141) on bone marrow core biopsies. IDO is different from most antibodies in that the antigen retrieval solution is...
12 April 2019 4,340 1 View
Immunohistochemistry or Immunohistological techniques for rat sample..
11 April 2019 9,310 2 View
Hi everyone! We are working with corneal endothelium on Descemet membrane. It can be considered as a cell monolayer on thin protein membrane, so you can treat it like a fresh tissue slide. We want...
10 April 2019 1,990 6 View
I am trying out immunofluorescence on rabbit spleen but the results are not fruitful due to auto-fluorescence. I tried blocking with 5%BSA for an hour still persists. I also tried sudan black but...
21 March 2019 1,697 8 View
I am using a flea cell flow system and need a hydrophobic border but sadly for the slides I am using for imaging they don't have the Teflon border. I was wondering if a PAP pen could be just as...
19 March 2019 339 2 View
Tropisms for various organ systems by borreliae have intrigued me for many years. In my research I have utilized autopsy tissues and tissue cultures for examination with Immunohistochemistry and...
18 March 2019 839 1 View
I am trying to stain for a particular protein in brain mouse tissue slices and am seeing a lot of background staining. The tissue was fixed with PFA. I generally block in goat serum for 1-hour at...
27 February 2019 1,782 6 View
Has anyone tried combining Immunohistochemistry using alkaline phosphatase technique and Immunofluorescence in the same sample slide?
01 February 2019 2,090 3 View
please, what molecules or hormones can I target with immunohistochemistry in the study of the impact of heavy metals on fertility? and what antibody can I use? And thank you in advance
17 January 2019 9,036 3 View