Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
Hello - I have been attempting to macrophages via IF with F4/80 in OCT-embedded mouse skeletal muscle using Thermo Rat Anti-Mouse monoclonal Ab (Cat# MF48000) with little success. I have been...
21 December 2019 4,988 3 View
We are processing clinical samples of bone marrow smears, and have encountered autofluorescence that makes subsequent FISH and IHC virtually impossible. The problems are: 1. the samples are of...
19 December 2019 4,878 4 View
I'm optimising the PSD-95 antibody 7E3 (Santa Cruz Biotechnology) to stain some 30um brain slices (resectioned from 300um). The current protocol I'm using has not been very successful in that I'm...
15 December 2019 9,049 1 View
I will work with paraffin tissue sections on the testicular tissue and for detection of apoptosis I will use TUNEL fluorescein kit (Biotium brand). 1) Do you recommend Biotium TUNEL kit? 2) The...
26 November 2019 6,199 4 View
Hello everyone, I am performing IHC for von Willebrand Factor on femurs and tibiae of 22-week-old SD rats. The bones are decalcified in EDTA and paraffin embedded. After antigen retrieval,...
20 November 2019 6,662 12 View
I'm studying the distribution of collagen I and II across the intevertebral disc of the Sprague Dawley tail using IHC. The fluorescent signals were supposed to vary from one another and appear...
13 November 2019 3,221 7 View
What is the best method for preparing gelatin methacrylate embedded cells for Immunofluorescence? I have fixed with 4% PFA, embedded in histogel, paraffin embedded and then performed antigen...
13 November 2019 9,261 0 View
I am new to the immunohistochemistry world and am currently about to teach myself the basics on some sections of C57BL/6 mouse lung. Does anyone have any good antibody recommendations or proteins...
29 October 2019 845 3 View
We are currently trying to phenotype the expression of a cell-adhesion molecule in Schwann cells and we would like to assess if there is differential expression of this molecule in myelinated and...
17 October 2019 3,781 2 View
I am developing an immunohistochemical protocol for an oxidative stress marker, but I am in doubt between the use of 1% BSA or 5% milk.
15 October 2019 6,155 2 View
Hello everybody, I tried to mark Dopamine beta hydroylase (DBH) in adult zebrafish, but I had no good results. I marked TH and DBH and it was just expressed TH in the tissue (postfixed with PFA...
04 October 2019 2,645 3 View
My cells are fixed on a coverslip and are stained with DAPI, Cytokeratin and CD45. What steps should I take after staining the cells?
19 September 2019 1,610 4 View
I have stained some sections of mouse intestinal epithelium for phosphorylated ERK. I would like to count the number of DAB positive cells, and express that as a % of the total number of cells...
11 September 2019 271 8 View
I am trying to quantify my images for a poster using ImageJ, but am having a hard time because they aren't white balanced. Is there a free application I can download which will allow me to white...
31 July 2019 3,321 5 View
.. My Rat spinal cord samples are preserved in sucrose 30% .. I cut the sections using cryostat at 40 micron, All my buffer solutions for 1ry & 2ry Antibodies, blocking solution, washes are...
26 July 2019 2,952 8 View
Does anyone know of any good hypothalamic nuclei markers that can be used for immunohistochemistry purposes?
18 June 2019 4,210 3 View
The endometrial biopsy was taken in the mid-lutheal phase of the menstrual cycle from a woman with recurrent implantation failure (RIF). Extremely high percentage of stromal and luminal epithelial...
12 June 2019 2,834 1 View
I'm trying free-floating IHC instead of slide-mounted for mouse brain sections and am currently trying to figure out the best blocking media for my purpose. Beyond something like an appropriate...
30 May 2019 458 4 View
Has anybody ever tried to perform antigen retrieval on frozen tissue sections by incubation in sucrose? We tried that in case of murine iNOS in the spinal cord and it works pretty nice. However, I...
29 May 2019 837 3 View
Does anyone have experience with AML12 cells and knows how to push the cells to synthesize lipid droplets? I am using DMEM/F-12 medium, 15% FBS and ITS-G(1:100) and still there is just a few...
21 May 2019 5,879 4 View
Hi guys. I keep getting staining on void area of tissue (breast tumor tissues). As you can see both in my negative control(without primary antibody) and positive control, there are stretch...
17 May 2019 8,506 5 View
I am currently doing immunohistochemistry to look at a membrane antigen, on fixed embedded samples. I want to amplify the signal (low) I am getting, and was wondering which kind of amplification...
25 April 2019 5,422 5 View
Is there a recommendation for a software/FiJi-ImageJ plugin/workflow for quantifying ratio of expression of a protein between nucleus and cytoplasm (or the nuclei and the rest of the photo) in...
25 April 2019 8,206 2 View
Both rabbit (anti-Sox2) and mouse (anti-PCNA) primary antibodies work in single IHC but not in a double IHC. This is true for a simple mix of both antibodies as well as a sequential IHC. This also...
23 April 2019 6,726 8 View