Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
Does anybody have any idea whether fixation protocol for IHC affects the post translational modification of antigens? Thank you in advance!
23 October 2017 3,777 2 View
Hi All, I need your comments, experiences and advice regarding how to count cell number of immunohistochemistry experiments by using image J ? or do you know any special software for counting...
06 October 2017 5,873 3 View
I have been testing new CD4, CD19 and IL-17 monoclonal antibodies for IHC that were made to work on formalin-fixed paraffin sections. These antibodies are from eBioscience and work really well in...
02 October 2017 1,092 2 View
In a lab I rotated in, we always reused primary antibody but never reused secondary. In the lab I've chosen to remain in, it is considered okay to reuse secondary antibodies. I am a bit hesitant...
28 September 2017 4,003 7 View
I am planning to perform macrophage immunohistochemistry in aortas from ApoE-deficient mice. Which is the best antibody and/or protocol to do it?
28 August 2017 4,371 5 View
Hello, I'm looking for a method to evaluate autoimmunity (disease-severity score) in B6/AIRE KO mice. I was advised to use immunohistochemistry of the eye, liver and salivary glands. However, I...
21 August 2017 4,564 2 View
I am doing IHC for normal proteins, but there must be a different protocol to treat phospho proteins. Please, enlighten me with that. Thanks!
03 August 2017 7,521 2 View
We used breast carcinoma tissue, running IHC used Leica Bond Max. The detachment tissue that we found is about 30%. All instrument (eg slide, antibody ER, PR) we used is from Leica. We don't...
03 August 2017 3,304 7 View
Hi, we are trying to localize the p16INK4a tumor supressor protein in neural cells from the murine brain (including the retina). We are looking for a good antibody for immunohistochemistry and/or...
02 August 2017 7,541 3 View
I've been conducting DAB stains for nuclear proteins, but I am consistently seeing an artifact like blurriness, as shown in Image 1. It's been largely at random, with only some slides experiencing...
27 July 2017 7,275 5 View
I have been using dyna magnetic beads from Invitrogen for my ChIP. I m also blocking beads overnight using salmon sperm DNA, BSA and glycogen. I have also tried blocking without Glycogen. But in...
14 July 2017 4,087 1 View
I am using Darpp32 Cell Signaling Ab, frozen rat brain sections. 1:200-1:300, DAB, have some issues with staining. It is already have an activation after 7 sec in DAB... May be some of you used...
05 July 2017 1,974 1 View
I have been preparing 10% EDTA solution for decalcification of rat knee joints that I want to use for IHC, and have been using it for 2.5 weeks. However, even though the pH may only change by 0.2...
30 June 2017 7,350 1 View
I am new to IHC and I am have not had success in staining with Bodipy 493/503 to get a clear image. I have attached an image to illustrate the problem that I am having. I have titrated the working...
27 June 2017 5,164 15 View
I am performing IHC with the ABC elite kits using Impact DAB, both from Vectorlabs. My problem is that the DAB solution precipitates when added to my intestine samples on the slide, like tinsel...
23 June 2017 3,066 3 View
Hello everybody!!! I overexpress an HA-tagged protein in the DG of the hippocampus (mice) by viral infection. Afterwards, i stain the brain slices against the HA tag by the IHC. As expected, I...
22 June 2017 6,139 3 View
We are trying to achieve better results in our immunohistochemistry assays (paraffin-embedded tissue sections) for antigen retrieval of surface markers such as CD3. Our current protocol of sodium...
09 June 2017 7,094 6 View
I'm looking for an immunohistochemistry protocol for Substance P (SP) in the Ventral Pallidum. I would appreciate any help.
01 June 2017 2,993 1 View
Hey everybody, does anyone have experience with kidney stainings (podocin) in embryos at 72 hpf? Wen want to image preferably the whole embryo without cutting afterwards. I'm really new to this...
22 May 2017 6,988 1 View
Hey team, hoping for some help here.The images look great, but I'm running into a roadblock for quantification of neurites in hippocampus, any suggestions?Details: I have several coronal rat brain...
17 May 2017 2,919 3 View
Hi, I want to do immunohistochemistry of GFAP in brain tissue (coronal sections). And this the first time I am doing IHC of mouse brain tissue. Normally for histopathology we take 5 micron...
11 May 2017 10,020 11 View
I've been seeing ring structures in mouse cortices for various different primaries (see ppt attached with GFAP, vWF, and Iba1). DAPI often appears in the center of these holes. The rings haven't...
08 May 2017 5,576 3 View
I would like to do some "proof of concept" immunofluorescence stainings on brain tissues and lymph nodes.
04 May 2017 6,415 3 View
We would like to analyze neurodegeneration by immunohistochemistry (or other tools such as FACS if they would be appropriate). Several techniques that are available including TUNEL, caspase3...
01 May 2017 1,582 11 View