Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
1,178 views 669 posts
Questions related to Immunohistochemistry
Hi everyone! We are working with corneal endothelium on Descemet membrane. It can be considered as a cell monolayer on thin protein membrane, so you can treat it like a fresh tissue slide. We want...
10 April 2019 2,001 6 View
I am trying out immunofluorescence on rabbit spleen but the results are not fruitful due to auto-fluorescence. I tried blocking with 5%BSA for an hour still persists. I also tried sudan black but...
21 March 2019 1,716 8 View
I am using a flea cell flow system and need a hydrophobic border but sadly for the slides I am using for imaging they don't have the Teflon border. I was wondering if a PAP pen could be just as...
19 March 2019 365 2 View
Tropisms for various organ systems by borreliae have intrigued me for many years. In my research I have utilized autopsy tissues and tissue cultures for examination with Immunohistochemistry and...
18 March 2019 858 1 View
I am trying to stain for a particular protein in brain mouse tissue slices and am seeing a lot of background staining. The tissue was fixed with PFA. I generally block in goat serum for 1-hour at...
27 February 2019 1,798 6 View
Has anyone tried combining Immunohistochemistry using alkaline phosphatase technique and Immunofluorescence in the same sample slide?
01 February 2019 2,114 3 View
please, what molecules or hormones can I target with immunohistochemistry in the study of the impact of heavy metals on fertility? and what antibody can I use? And thank you in advance
17 January 2019 9,046 3 View
I have brain cryosections on gelatin coated slides. I keep them in -20 or -80. For IHC, I dry the sections for 30 min at room temperature, wash them for 15 min with PBS and start antigen retrieval...
16 January 2019 2,192 0 View
HI, i am having difficulties with staining for Phospho TAU in KA-induced animal model. I am using free floating immuno histochemistry method. I am using AT-270, At-180, AT-100, AT-08 antibody...
15 January 2019 2,921 4 View
we need to hear from the experts their experience on the field. thanks
09 January 2019 3,651 4 View
Hi I have a question in setting up a control for background staining when doing IHC. I have a polyclonal antibody to stain tissue using IHC. I was going to use isotype control. However, it seems...
09 January 2019 2,970 3 View
l need to assess the potential effect of certain drug on NF-kB.
02 January 2019 4,406 3 View
Which temperature is optimal for storage? Shall I fix slides before storage?
11 December 2018 728 4 View
I perfused the mice, fixed the brains and used the Microslicer to cut to slides then used the avidin-biotin-peroxidase (ABC method) to do immunohistochemistry analysis. the method for details I...
18 November 2018 6,191 2 View
Hi everyone. We want to do an IHC in peripheral blood smear but it's our first time, and we have some doubts. How do we have to fix the samples? Which is the best fixation agent, acetone, methanol...
16 October 2018 9,492 5 View
Hi all. I'm using a biotinylated conotoxin to attempt to visualise voltage gated calcium channels. At the moment I'm getting such high background/non-specific staining that it's impossible to see...
15 October 2018 6,730 3 View
I need good antibodies for alpha1A and alpha1D adrenergic receptors. I've been using one from abcam that is claimed to bind to both subtypes, but it didn't work too well. Moreover, after I've done...
29 September 2018 755 1 View
Our group is doing OCT frozen section IHC and neurofilament staining for nerve fiber count and density analysis. Unfortunately our rat facial nerve tissue appears to all suffer from severe...
24 September 2018 7,834 2 View
I want to localize the pinealocytes, using immunohistochemistry. Generally, the pineal organ of the catfish is very tiny and thread like appearance. Sectioning of the pineal organ is too...
21 September 2018 7,983 9 View
I am trying to stain the protein that regulates the cysteine levels in the different organs (human normal colon in my case). there seems to be equal amount of background in the isotope control...
10 August 2018 1,492 6 View
We're looking to label the arteries and veins separately within mouse brain tissue but are unsure how to do that. Does anyone have knowledge about how to go about doing this, such as which...
30 July 2018 9,798 7 View
I've got blocks of rat paw skin that have been immersion fixed in 4% PFA for 16 hours, then washed and cryoprotected in 30% sucrose. I ideally need to store these for about 6 months, before...
23 July 2018 9,417 8 View
I just want to make sure about some things : 1. I knew that xylene is used to remove the paraffin 2. Ethanol in various concentrations are for rehydration (why the tissue should be rehydrate?) and...
07 July 2018 2,502 5 View
I will be doing some mouse retinal whole mounts and want to label microglia in both resting/ramified and activated states. I have an option of using either a goat Iba-1 or a rat CD11b. I would...
27 June 2018 5,400 3 View