Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
HI, i am having difficulties with staining for Phospho TAU in KA-induced animal model. I am using free floating immuno histochemistry method. I am using AT-270, At-180, AT-100, AT-08 antibody...
15 January 2019 2,902 4 View
we need to hear from the experts their experience on the field. thanks
09 January 2019 3,640 4 View
Hi I have a question in setting up a control for background staining when doing IHC. I have a polyclonal antibody to stain tissue using IHC. I was going to use isotype control. However, it seems...
09 January 2019 2,963 3 View
l need to assess the potential effect of certain drug on NF-kB.
02 January 2019 4,397 3 View
Which temperature is optimal for storage? Shall I fix slides before storage?
11 December 2018 706 4 View
I perfused the mice, fixed the brains and used the Microslicer to cut to slides then used the avidin-biotin-peroxidase (ABC method) to do immunohistochemistry analysis. the method for details I...
18 November 2018 6,177 2 View
Hi everyone. We want to do an IHC in peripheral blood smear but it's our first time, and we have some doubts. How do we have to fix the samples? Which is the best fixation agent, acetone, methanol...
16 October 2018 9,465 5 View
Hi all. I'm using a biotinylated conotoxin to attempt to visualise voltage gated calcium channels. At the moment I'm getting such high background/non-specific staining that it's impossible to see...
15 October 2018 6,717 3 View
I need good antibodies for alpha1A and alpha1D adrenergic receptors. I've been using one from abcam that is claimed to bind to both subtypes, but it didn't work too well. Moreover, after I've done...
29 September 2018 739 1 View
Our group is doing OCT frozen section IHC and neurofilament staining for nerve fiber count and density analysis. Unfortunately our rat facial nerve tissue appears to all suffer from severe...
24 September 2018 7,828 2 View
I want to localize the pinealocytes, using immunohistochemistry. Generally, the pineal organ of the catfish is very tiny and thread like appearance. Sectioning of the pineal organ is too...
21 September 2018 7,971 9 View
I am trying to stain the protein that regulates the cysteine levels in the different organs (human normal colon in my case). there seems to be equal amount of background in the isotope control...
10 August 2018 1,481 6 View
We're looking to label the arteries and veins separately within mouse brain tissue but are unsure how to do that. Does anyone have knowledge about how to go about doing this, such as which...
30 July 2018 9,793 7 View
I've got blocks of rat paw skin that have been immersion fixed in 4% PFA for 16 hours, then washed and cryoprotected in 30% sucrose. I ideally need to store these for about 6 months, before...
23 July 2018 9,401 8 View
I just want to make sure about some things : 1. I knew that xylene is used to remove the paraffin 2. Ethanol in various concentrations are for rehydration (why the tissue should be rehydrate?) and...
07 July 2018 2,497 5 View
I will be doing some mouse retinal whole mounts and want to label microglia in both resting/ramified and activated states. I have an option of using either a goat Iba-1 or a rat CD11b. I would...
27 June 2018 5,396 3 View
I am currently processing 400 um hippocampal slices immunohistochemically. I have not had too much trouble with my images and antibody binding. That said, I am aware that over fixation may...
23 June 2018 9,688 5 View
I'm looking for an antibody (preferably validated) for growth hormone releasing hormone (GHRH/GHRF) for immunohistochemistry in rat brains. Do you have any leads? I would really appreciate your...
19 June 2018 5,446 1 View
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12 June 2018 4,993 4 View
I've seen various protocols suggesting either drying samples on a heat-block, dehydrating through an increasing EtOH concentration gradient or just air-drying before mounting or storage. What are...
04 June 2018 7,208 3 View
I need to detect p53 in murine E15.5 embryos that overexpress this protein. The embryos were collected and fixed overnight in 10% Neutral Buffered Formalin. Then, I cryopreserved these embryos in...
24 May 2018 2,704 2 View
Hello everyone, I have stained brain sections of Zebrafish using Nissl staining and I watched cracks in the tissue :B . It's quite disturbing. Any help will be appreciated. Thanks :)
22 May 2018 1,981 8 View
We took images at 10X of tissue from the central nucleus (CeA) of the amygdala, but those images included tissue outside of the CeA. In order to simplify things and keep from having to re-image...
11 May 2018 10,132 4 View
Hi, I am testing antibodies by immunohistochemistry (IHC) to subsequently use them for immunoprecipitation (IP) of the proteins in question. Now I did come across two problems: 1. My antibody...
28 April 2018 2,069 5 View