Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
I am currently processing 400 um hippocampal slices immunohistochemically. I have not had too much trouble with my images and antibody binding. That said, I am aware that over fixation may...
23 June 2018 9,719 5 View
I'm looking for an antibody (preferably validated) for growth hormone releasing hormone (GHRH/GHRF) for immunohistochemistry in rat brains. Do you have any leads? I would really appreciate your...
19 June 2018 5,469 1 View
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12 June 2018 4,998 4 View
I've seen various protocols suggesting either drying samples on a heat-block, dehydrating through an increasing EtOH concentration gradient or just air-drying before mounting or storage. What are...
04 June 2018 7,217 3 View
I need to detect p53 in murine E15.5 embryos that overexpress this protein. The embryos were collected and fixed overnight in 10% Neutral Buffered Formalin. Then, I cryopreserved these embryos in...
24 May 2018 2,728 2 View
Hello everyone, I have stained brain sections of Zebrafish using Nissl staining and I watched cracks in the tissue :B . It's quite disturbing. Any help will be appreciated. Thanks :)
22 May 2018 1,998 8 View
We took images at 10X of tissue from the central nucleus (CeA) of the amygdala, but those images included tissue outside of the CeA. In order to simplify things and keep from having to re-image...
11 May 2018 10,150 4 View
Hi, I am testing antibodies by immunohistochemistry (IHC) to subsequently use them for immunoprecipitation (IP) of the proteins in question. Now I did come across two problems: 1. My antibody...
28 April 2018 2,073 5 View
Dear all, Anybody could explain to me which method is better for measurement the level of toll like receptor protein in tumor tissue , Immunohistochemistry vs western blot? Thanks in advance.
10 April 2018 6,465 5 View
I have been staining mouse tissue using primary antibodies raised in mouse, trying to detect microglia using an anti-CD45 antibody (the best marker we had available) and a membrane-bound protein...
30 March 2018 9,827 3 View
Dear fellow scholars, We have a mouse line with eGFP and tdTomato labeled axons going from the brainstem to the spinal cord. We are going to clear the spinal cord (using iDisco) in embryos and...
22 March 2018 471 2 View
Breast samples I get for an IHC study are mostly autolyzed. I choose well preserved sections carefully, but when I get unusual result, can't decide whether it's a finding or artefact. e.g....
15 March 2018 6,321 3 View
Hello everyone, I am attempting to stain the trabecular bone of proximal tibia and cortical bone of the midshaft tibia. Tissue was fixed in formalin for approx. 4 months followed by...
07 March 2018 2,482 4 View
I am doing IHC with bone and cartilage samples (eg. growth plate). For antigen retrieval, I usually heat the samples in Citrate buffer (pH 6.0) using pressure cooker (for 1 min at max. temp and...
26 February 2018 4,840 8 View
I'm planning on doing a combined staining on FFPE TMA slides: ISH (red dots) and a nuclear IHC in DAB (brown nuclei). We would like to also visualize the collagen in this same staining. However,...
22 February 2018 8,691 3 View
MUC1 staining (DAB) has been successful for IHC, both with tris and citrate buffers, however, western blot have failed miserably. CST VU4H5 antibody is compatible for both IHC and WB. Is...
12 February 2018 7,355 10 View
Hi, I have IHC staining on 2-3 serial FFPE tissue sections. I did my best to take pictures of the same regions on a brightfield microscope but I'd like to be able to align each section into a...
07 February 2018 3,525 5 View
Hi everybody, I'd like currently to evaluate virus-induced NLPR3 inflammasome activation both in vitro and in vivo. For in vitro analysis by Western Blot, there is no problem. However, I did not...
01 February 2018 8,523 3 View
Identification and quantification of senescent cells in human tissues is a challenging task. It is well known that the application of a single biomarker such as SA-b-gal activity (the most widely...
23 January 2018 9,144 4 View
I rely on numerous articles, however, in regard to the removal of the historesin, I face some difficulty. When we talk about removal of historesin, would this resin be completely removed? Or is...
04 January 2018 1,734 2 View
I am using the Abcam antibody ab4680 on mouse brain tissue fixed via PFA perfusion. The protocol we are using is the same as in http://dx.doi.org/10.1016/j.cell.2015.11.025. It's a staining method...
24 December 2017 7,149 3 View
Hi! I have purchased Nrf2 Abcam antibody (ab31163). I want to perform an inmunohistochemistry in paraformaldehyde fixed rat brain sections. I have not clear which protocol should I follow,...
11 December 2017 1,452 3 View
We're using diaminobenzidine (DAB) to visualize staining on slide-mounted paraffin-embedded mouse embryonic tissue. Often, large parts of the tissue won't stain, like a boundary is just drawn...
07 December 2017 6,611 4 View
If the mounting is delayed for a day or two, would the specific binding being greatly reduced?
06 December 2017 4,254 3 View