Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
Dear all, Anybody could explain to me which method is better for measurement the level of toll like receptor protein in tumor tissue , Immunohistochemistry vs western blot? Thanks in advance.
10 April 2018 6,441 5 View
I have been staining mouse tissue using primary antibodies raised in mouse, trying to detect microglia using an anti-CD45 antibody (the best marker we had available) and a membrane-bound protein...
30 March 2018 9,823 3 View
Dear fellow scholars, We have a mouse line with eGFP and tdTomato labeled axons going from the brainstem to the spinal cord. We are going to clear the spinal cord (using iDisco) in embryos and...
22 March 2018 455 2 View
Breast samples I get for an IHC study are mostly autolyzed. I choose well preserved sections carefully, but when I get unusual result, can't decide whether it's a finding or artefact. e.g....
15 March 2018 6,303 3 View
Hello everyone, I am attempting to stain the trabecular bone of proximal tibia and cortical bone of the midshaft tibia. Tissue was fixed in formalin for approx. 4 months followed by...
07 March 2018 2,474 4 View
I am doing IHC with bone and cartilage samples (eg. growth plate). For antigen retrieval, I usually heat the samples in Citrate buffer (pH 6.0) using pressure cooker (for 1 min at max. temp and...
26 February 2018 4,826 8 View
I'm planning on doing a combined staining on FFPE TMA slides: ISH (red dots) and a nuclear IHC in DAB (brown nuclei). We would like to also visualize the collagen in this same staining. However,...
22 February 2018 8,679 3 View
MUC1 staining (DAB) has been successful for IHC, both with tris and citrate buffers, however, western blot have failed miserably. CST VU4H5 antibody is compatible for both IHC and WB. Is...
12 February 2018 7,348 10 View
Hi, I have IHC staining on 2-3 serial FFPE tissue sections. I did my best to take pictures of the same regions on a brightfield microscope but I'd like to be able to align each section into a...
07 February 2018 3,501 5 View
Hi everybody, I'd like currently to evaluate virus-induced NLPR3 inflammasome activation both in vitro and in vivo. For in vitro analysis by Western Blot, there is no problem. However, I did not...
01 February 2018 8,519 3 View
Identification and quantification of senescent cells in human tissues is a challenging task. It is well known that the application of a single biomarker such as SA-b-gal activity (the most widely...
23 January 2018 9,121 4 View
I rely on numerous articles, however, in regard to the removal of the historesin, I face some difficulty. When we talk about removal of historesin, would this resin be completely removed? Or is...
04 January 2018 1,729 2 View
I am using the Abcam antibody ab4680 on mouse brain tissue fixed via PFA perfusion. The protocol we are using is the same as in http://dx.doi.org/10.1016/j.cell.2015.11.025. It's a staining method...
24 December 2017 7,126 3 View
Hi! I have purchased Nrf2 Abcam antibody (ab31163). I want to perform an inmunohistochemistry in paraformaldehyde fixed rat brain sections. I have not clear which protocol should I follow,...
11 December 2017 1,446 3 View
We're using diaminobenzidine (DAB) to visualize staining on slide-mounted paraffin-embedded mouse embryonic tissue. Often, large parts of the tissue won't stain, like a boundary is just drawn...
07 December 2017 6,606 4 View
If the mounting is delayed for a day or two, would the specific binding being greatly reduced?
06 December 2017 4,233 3 View
I would like to stain for Epidermal Langerhans cell in rat skin. The problem is I am not sure what primary antibody would be the most appropriate for immunohistochemistry. I currently have CD103...
30 November 2017 4,337 3 View
Hi all, I am trying to combine 4 different markers for my multiplexed IHC. Their Antigen Retrieval (AR) conditions are as follows: Marker A: pH9 microwave, 30 mins Marker B: pH9 microwave, 15...
27 November 2017 6,119 3 View
Our lab is looking to measure mitochondrial dysfunction in spinal cord tissue. We think that a drug is reducing mitochondrial damage normally caused by experimental autoimmune encephalomyelitis....
31 October 2017 9,046 3 View
So...I am running immunohistochemistry assays of ovarian cancer tissues that are fixated and prepared by someone else (same person every time). Lastly, between different runs with the auto stainer...
30 October 2017 2,313 6 View
Hi all, I have a Knock out mouse' spinal cord (SC) fixed in tissue tek, and it was treated before with PFA, and sucrose (for using it in IHC experiment). However, I need to do a genotyping...
30 October 2017 2,581 10 View
Concerning our master thesis, we are evaluating immunohistochemical stained slides from the canine myometrium. We have earlier worked with the old program Imagetool, quantifying our images by...
30 October 2017 6,892 3 View
My tissue is a mouse tissue and my primary antibody also mouse. I have already tried mouse on mouse kit. ı have still staining at negative control. My tissue have parafin sections. Can anyone...
30 October 2017 7,623 9 View
I prepared, separately, rabbit anti-rat P4HB (1:50 dilution) and rabbit anti-rat CD68 (1:100 dilution) primary antibodies in 10% normal goat serum (in TTBS w/ 0.05% Triton-X 100, as per our usual...
24 October 2017 1,206 4 View