Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
1,008 views 654 posts
Questions related to Immunohistochemistry
Hi there, is there a cholesterol efflux assay kit suitable for suspension cells (I use Jurkat cells specifically)? Or has anyone used these kits for suspension cells?...
22 March 2023 7,263 0 View
We would like to use LPS for microglial activation (M1 polarization). These are iPSC-derived microglia and are relatively less sensitive compared to monocytes or macrophages. I see a lot of...
03 January 2023 8,777 2 View
I would like to know if it is possible and if yes how to store cryosections which were fixed with 4% PFA after sectioning? I only find protocols which immediately go on with the staining procedure...
22 October 2022 542 6 View
Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...
04 February 2021 3,272 1 View
Hi, I am trying to perform an IHC targetting a protein tagged with an Histidine tail. For this reason we ordered a recombinant Anti-6X His tag® antibody, which brings the following dilution...
01 February 2021 8,826 3 View
Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...
06 January 2021 5,587 1 View
Current protocol does not give clear images.
05 January 2021 1,749 3 View
Dear Collegues, If we want to perform immunohistochemistry /immunofluorence staining from FFPE human biopsy (vein) samples and study the presence of LPS and or common pathogen like Porphyromonas...
02 December 2020 5,826 2 View
Hi guys, I m wondering to know my pAb binding sites to the antigen. I am not sure about my pAb's binding towards N terminal or C terminals of antigen. How I can check this? Also, does molecular...
27 October 2020 2,554 2 View
I have been carrying out IHC to stain FLAG in fresh-frozen rat brain using FLAG M2 (F1804) with ABC then DAB, but am unable to identify positive staining, and seeing a high background. I carried...
09 October 2020 9,061 2 View
Dear colleagues, In my immunofluorescence stainigs I use liquid blocker pen, before solution with 0.3% Triton X-100. This helps to avoid dripping of solutions with primary antibodies and Triton...
01 October 2020 9,269 4 View
I am planning an experiment where I've to detect cells which are expressing TetR, my vector is so big that I can't have a reporter in it. I was thinking if I can detect the expression of TetR...
25 September 2020 1,203 4 View
I wish to estimate the expression of two types of markers by immunohistochemistry in the biopsy specimens of a particular type of cancer, and correlate their expressions with clinical parameters...
21 September 2020 9,798 6 View
I am looking to complete western blot experiments and IHC experiments on the same cohort of mice. Is it possible to Harvest the mouse and use the right hemisphere for IHC and the left for western...
09 September 2020 5,564 3 View
Hi everyone, we are still looking for good antibody for western blot and immunohistochemistry for the PXR both mouse and human.
08 September 2020 9,502 2 View
Hi everyone, we are still looking for good antibody for western blot and immunohistochemistry for the GPBAR-1 (TGR5) in both mouse and human.
08 September 2020 9,357 2 View
I have 3 groups of animal apart from PBS controls where i treat them under different immunosuppressive conditions. when the animals are subjected to partial or no immunosuppressive conditions, i...
01 September 2020 9,114 5 View
I am planning a double antibody ICC on hepatocytes using polyclonal antibodies; however, one of the primary antibodies I want to use is only verified for use in IHC. Can I adapt it to be used in...
17 August 2020 4,230 3 View
I want to know how to look/methods of(analyze and scoring) in different parts pathological section like stroma, Ductal epithelial, epithelial lining, luminal epithelial etc. in a different...
12 August 2020 1,118 4 View
Looking to perform IHC co-staining with CD3 (cytoplasmic) and MUM1 (nuclear) antibodies. CD3 polyclonal rabbit, HIER Na citrate pH6. MUM1 is monoclonal mouse, HIER EDTA pH9. I've tried HIER on...
02 August 2020 562 2 View
In IHC for double staining i use DAB and AP. DAB works fine but AP after mounting is a headache as i see precipitates and also sometimes dissolves.I have tried drying the slides a bit and then...
27 July 2020 341 2 View
So, basically I would like to analyze the entire Hippocampus concerning the number of cells showing a certain stain. However, with the magnification I need to differentiate between the cells, the...
24 July 2020 471 2 View
I accidentally left my tissue (mouse pancreas/duodenum/lung/spleen) in PFA overnight instead of 30% sucrose. Typically, I fix the tissue in 4% PFA for 45-60 minutes and then 30% sucrose O/N. I...
07 July 2020 4,195 0 View
I am working with rabbit spleen and as it is difficult to obtain antibodies against rabbits as most of them are raised in rabbits. I had to purchase a few antibodies raised in rabbits. But as...
18 June 2020 4,027 8 View