Histochemical localization of immunoreactive substances using labeled antibodies as reagents. | Contact experts in Immunohistochemistry to get answers
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Questions related to Immunohistochemistry
I want to know how to look/analyze different parts in a tumor section like stroma, Ductal epithelial, epithelial lining, luminal epithelial etc etc in a breast cancer tissue slide. Also is there...
03 June 2020 5,174 3 View
Hello! I am looking for good antibodies that help in clearly distinguish between D1- and D2-type medium spiny neurons (MSNs) in the striatum, by immunohistochemistry and immunofluorescent...
01 June 2020 5,117 3 View
Any Immunohistochemistry markers?? Thanks in advance.
31 May 2020 6,333 10 View
Hi! I'm analyzing some TMA IHC cores using an scoring method that combines intensity and proportion of stained cells. I would like to know if is there any protocol to quantify percentage of DAB...
26 April 2020 578 2 View
When I am planning my experiment I would like to check both the c Fos level and p ERK level. But on literature review, I found that c fos and p ERK is determined by immunohistochemistry but the c...
18 March 2020 2,098 3 View
I need to fix the Adipose tissue from Mice for IHC. I am looking for a good protocol to follow.
09 March 2020 8,452 3 View
Hi. I am doing immunohistochemistry staining on mice tissue. We perfuse the mice and place the brain in 4% PFA O/N then switch to 48hr in 30% sucrose (or until the brain sinks). However I am...
05 March 2020 2,827 9 View
Does anyone have protocol recommendations for freezing/storing human uterine tissues? I have seen some protocols that place tissues directly in LN2 and then transfer to -80 for long term storage....
03 March 2020 3,431 2 View
I have spleen samples from infected mice which are currently frozen in OCT. Ultaimtely I would like to use immunohistochemistry to image spleen slices and see the ROS within different...
28 February 2020 4,534 3 View
Hello, I saw in some papers that this article was cited and I want to reach it, but I could not find it anywhere. Here is the citation I saw: Miyazawa T, Iino Y, Murakami Y, Ishii T (2000)...
21 February 2020 5,436 3 View
CST and DBS PD-L1 is not working for me. Can any one suggest a better option?
30 January 2020 8,386 10 View
I inherited a staining protocol from another lab. It specifically says not to swirl the ABC mixture, but instead to let it sit for 30 minutes before adding the tissue. It does not specify if the...
27 January 2020 5,936 2 View
Hello everyone, Recently, I have bought one of the biolegend company products named “Purified anti-human CD56 (NCAM), Clone: MEM-188”, and realized this product is validated for...
27 January 2020 3,852 3 View
I would be glad if you help to me.
22 January 2020 6,404 2 View
I have human eye cryosections that I performed immunohistochemistry on . The issue I'm having is visualizing my antibodies in the retinal pigment epithelium layer because it has an...
20 January 2020 4,288 3 View
Hi, (a) I am trying to analyze histological scores of more than 3 groups, each having 6 animals. Which statistical tool is the appropriate one? I checked normality which appears to be of mixed...
17 January 2020 4,735 3 View
Dear colleagues I am planning to use anti-caspase 3 active in frozen brains. Have you performed that? Because I only find when tissues are paraffin-embedded. i will appreciate your answer and...
17 January 2020 5,780 2 View
Hi everyone, In case that, it is difficult to get animal serum from commercial vendors. Have you ever tried to collect animal serum to be used for blocking in immunohistochemistry ? If so do we...
05 January 2020 3,755 5 View
We are processing clinical samples of bone marrow smears, and have encountered autofluorescence that makes subsequent FISH and IHC virtually impossible. The problems are: 1. the samples are of...
19 December 2019 4,785 4 View
I'm optimising the PSD-95 antibody 7E3 (Santa Cruz Biotechnology) to stain some 30um brain slices (resectioned from 300um). The current protocol I'm using has not been very successful in that I'm...
15 December 2019 8,972 1 View
I will work with paraffin tissue sections on the testicular tissue and for detection of apoptosis I will use TUNEL fluorescein kit (Biotium brand). 1) Do you recommend Biotium TUNEL kit? 2) The...
26 November 2019 6,150 4 View
Hello everyone, I am performing IHC for von Willebrand Factor on femurs and tibiae of 22-week-old SD rats. The bones are decalcified in EDTA and paraffin embedded. After antigen retrieval,...
20 November 2019 6,607 12 View
I'm studying the distribution of collagen I and II across the intevertebral disc of the Sprague Dawley tail using IHC. The fluorescent signals were supposed to vary from one another and appear...
13 November 2019 3,152 7 View
What is the best method for preparing gelatin methacrylate embedded cells for Immunofluorescence? I have fixed with 4% PFA, embedded in histogel, paraffin embedded and then performed antigen...
13 November 2019 9,194 0 View