I understand that autofluorescence is particularly problematic with placental and fetal membrane tissue - are there ways around this, or should I stick with peroxidase secondary antibodies?
Jennifer,
Speaking generally, since there is a large number of fluorophores available, even if you have issues with autofluorescence in one part of the spectrum (say FITC), it should be possible to pick one where autofluorescence is less prevalent or absent, especially when you combine it with narrow band filters.
Since you mentioned Peroxidase as an option, I take it that you are not concerned about concurrent detection of multiple antigens. If so, and your target is relatively abundant, I would tend to favor Peroxidase based detection. The resultant product tends to be much more stable and slides easy to store/archive.
I searched on SciGine and found the attached method for IHC of placental tissue from humans. Seems like a perfect match for your question :)
http://www.scigine.com/method.php?ID=1274
Jennifer,
Speaking generally, since there is a large number of fluorophores available, even if you have issues with autofluorescence in one part of the spectrum (say FITC), it should be possible to pick one where autofluorescence is less prevalent or absent, especially when you combine it with narrow band filters.
Since you mentioned Peroxidase as an option, I take it that you are not concerned about concurrent detection of multiple antigens. If so, and your target is relatively abundant, I would tend to favor Peroxidase based detection. The resultant product tends to be much more stable and slides easy to store/archive.
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