Any suggestions for CD86 antibodies for IF with PFA fixed mouse tissue? Can mouse monoclonal antibodies be used on mouse tissue?

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Torsten Goldmann

For mouse on mouse you need a mouse on mouse kit.

Try this link: http://www.abcam.com/protocols/mouse-on-mouse-staining-protocol

Best

Torsten

Delaney Caroline Swindle

Thank you Torsten! Very helpful!

Russell Linscott

There are also a large number of non-mouse Abs out there (mostly Rabbit pAbs and Rat mAbs). If you want some sources to explore:

http://www.linscottsdirectory.com/search/antibodies/searchStr:CD86

You can select "Mouse" from the Reactive Species filter. Follow the More Info links to the suppliers' data pages for more details. Good luck.