Anyone knows if the procedure of deparaffinization can alter the structure/composition of plasma membranes?
If the organic solvents of deparaffination have an impact on plasma membranes, the same reagenses in the paraffin-process do the same job. So if you are concerned about the effect of organic solvents on lipids in the plasma-membrane, you have also to look at the steps before. Lipoproteins should be preserved by a sufficient formalin-fixation via the protein-part of the molecule.
As mentioned by Gudrun Lang also, the cellular structural integrity in the section would depend more upon the fixation step. As long as you are not doing something out of the ordinary, post-fixation processing should not impact the tissue/cells much. De-paraffinization using Xylene solution (3X5 minutes each) is a pretty standard process employed by majority of the labs worldwide.
Yes. Deparafinization more that 5 min may cause it. But I agry with previos answers of collegues. unadequate fixation more often causes it.
Thank you everyone. As we are interested in studying membrane composition, most probably we would need to use another biospecimen such as frozen tissues?
Good day, Claudia. You may use so method for control of dearafinization: add to your test samples slides, slide with section of lymph node with reactive hyperplasia. Than make IHC in stainer, but stain limph node for Ki-67(MIB). If deparafinization made correct you will see stainig in lymp node.
In a case, when you make IHc protocol manually you can mount lyph node section direct test samples at the same slide.
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