Does anybody Knows, a protocol for GFAP Immunohistochemistry?

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Karthik Raman

Hey there, the attached link talks about a method for immunohistochem for GFAP in a fetal mouse brain. There are lots a great methods on immunohistochemistry on SciGine.

http://scigine.com/method.php?ID=4453

Refik Kanjhan

Dear Ricardo,

I have used GFAP labelling alone or in combination with others such vimentin in brain slices, retina and cochlear tissues. For details please see my following publications.

best wishes, Refik

Article Postnatal developmental expression of the PDZ scaffolds Na+/...

Article The two-pore domain K+ channel TASK-1 is closely associated ...

Jill Miotke

I have had good luck with the Dakopatts polyclonal anti-GFAP (rabbit anti-cow GFAP), product number Z0334. It reacts with GFAP in a wide variety of mammalian species. I have labeled astrocytes in culture and in frozen sections of paraformaldehyde-fixed tissue.

I have also used the monoclonal anti-GFAP clone G-A-5. Many companies have this clone, so I would look to see who might have either a trial size or has good ratings for the product.

Good Luck!

Jill