Immunohistochemistry
I have no experience in this regard
INS-RTK modules claim to be close to a cm accuracy, has anyone used one and if so, how expensive can the telematic charges be if I have a case to run it 18h per day?
14 December 2020 6,139 8 View
Hello everyone, Can anyone kindly explain what is mean by three different colours of cracks in RC beams in ANSYS APDL and the features of first crack (red colour outline) second crack (green...
17 November 2020 2,512 1 View
My RC beams experimental ultimate load & deflection was 97kN and 20mm resp. But when I model it in ansys apdl it run continuously up to a load of 140kN and 60mm deflection and still...
02 October 2020 8,747 2 View
I am analyzing RC beam in APDL and tried convergence criteria with F=0.05 once then U=0.05 and also tried both criterias simultaneously but what I found is my solution continuous even upto twice...
02 October 2020 1,490 2 View
Can anyone kindly explain in case of Hognestad compressive stress-strain curve for concrete, they have given fc" =0.85fc', but why researchers are use fc"=fc' instead. Where fc" =ultimate...
14 August 2020 7,508 3 View
Dear researchers, Greetings! Please help me to study surface of Fe(110) with Organic Corrosion inhibitor molecules using Materials Studio software. Kindly give suggestion to create a simulation...
02 August 2020 4,267 2 View
12 March 2020 6,331 14 View
I have an ordinal dataset on the scale of 1 to 5 based on household surveys about the intensity of agreement (5 being strongly agreed to 1 being strongly disagreed) of residents about certain...
11 March 2020 6,308 2 View
28 August 2019 2,091 6 View
good morning everyone, i need your help once again, As i am analysing RCC Simply supported beam in ANSYS APDL. i run the model upto ultimate failure, now i want to check the stresses in steel bars...
12 June 2019 7,168 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...
07 February 2021 8,457 1 View
Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...
03 February 2021 3,039 1 View
31 January 2021 8,678 3 View
Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...
21 January 2021 9,826 3 View
Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...
10 January 2021 4,002 5 View
Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...
05 January 2021 5,420 1 View
Current protocol does not give clear images.
04 January 2021 1,614 3 View
Immunohistochemistry staining. Tissue Fixation during IHC. Using DAB. blocking step. controls group.
23 December 2020 5,768 2 View
Suggestions and recommendations welcome. Any usage/protocol experience would also be helpful/appreciated. Cheers
22 December 2020 599 3 View