Immunohistochemistry
I have no experience in this regard
I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...
18 July 2024 2,881 4 View
Hi Everyone, I am working on a research project focused on analyzing behavior and cognition in different environments and situations. I am looking for a solution that allows us to: Record...
29 June 2024 6,540 0 View
1. Which phase should I select during EBSD? 2. Attached image is the polished surface but in your opinion what is on the surface of sample? it is oxide or colloidal? 3. Diamond polishing by paste...
27 June 2024 5,248 2 View
I had polished my sample till 3000 grade manually then done diamond polishing by 3 micron, 1 micron and 1/4 micron (By Diamond paste and aerosol). After that I had done colloidal polishing by 0.04...
19 June 2024 5,343 3 View
For beginner researcher, authors are confused to submit a paper on conference or journal publication in the majority times. I would like to give insight opinions from it.
11 June 2024 6,602 13 View
Dear Professors, If any one know about the technical conference in Australia between 14-21 September 2024, Please let me know. Thanking you in advance.
09 June 2024 3,867 1 View
I have used Keller's reagent till now but I am not able to see silicon network in SEM. I am also facing problem in etching. Some times I can see the microstructure in Optical Microscope but not...
13 March 2024 9,866 1 View
Please mention the some secondary data methods.
10 January 2024 2,305 3 View
I am looking for TP53 status of various carcinoma cell line from DepMap portal (https://depmap.org/portal/gene/TP53?tab=characterization&characterization=mutation), but could not get the P53...
03 January 2024 1,643 0 View
I want to quantify the CO2 uptake for a hempcrete panel (Eg: 5 years) any instrumental methods or any other methods to find this data?
04 November 2023 5,248 0 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
Hi, I am dealing with plant parasitic nematodes. I tried extracting RNA from almost 1000J2 worms with two different kits (Qiagen RNeasy Kit, Omega RNA extraction Kit) and by two different ways of...
09 July 2024 3,742 2 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...
25 June 2024 7,825 2 View
Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...
05 June 2024 624 2 View
I need to do immunohistochemistry in mouse brain, particularly in neurotensin-cre mouse line.
22 May 2024 3,565 7 View
Hi all, I've processed my own IHC for many years and never run into this problem before, but the last few times I've tried staining, I've had the same issue. I'm wondering if it's a reagent...
08 May 2024 3,979 1 View
