I work with microglial cells of difficult adhesion, can't that adhere to any support. so far deal with a coating of gelatin, collagen, poly-d-lysine. Anyone have any other idea of some strong coating for adherer my cells in which does not have much background noise, since I use the cells for performing immunohistochemistry.
Many thanks and sorry for my English.
We used for neurons that are difficult to growth in culture poly-DLysine (10 micrograms/ml) petri dishes at a density of 1.5X105 cells/cm2. and worked very well.
Microglia usually adhere very strongly to almost anything. Do your cells have some kind of mutation? I also agree with the above. I have not known any cell type that did not adhere strongly to Matrigel. Just make sure that you you stain at room temperature if the cells and substrate are not well fixed. If you need to use a very light fixation for your antibodies the Matrigel can re-liquify if you stain at 4 desrees C.
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