We would like to use LPS for microglial activation (M1 polarization). These are iPSC-derived microglia and are relatively less sensitive compared to monocytes or macrophages.
I see a lot of different LPS strains available through the Sigma Aldrich website:
O111:B4
O26:B6,
O55:B5,
O127:B8,
O128:B12
1. Would anyone happen to know how these LPS strains compare in potency? Which strain is most potent for inducing M1 state?
2. In my reading, O111 and O55 are the most commonly used strains for M1 polarozation? Does anyone know how they compare in potency?
Thank you.
hi
Lipopolysaccharide (LPS) is a component of the outer membrane of gram-negative bacteria and is known to stimulate the immune system. When LPS is introduced into the body or added to cells in culture, it activates the immune system and can lead to the production of cytokines and other immune mediators.
LPS is commonly used in research to stimulate the immune system and study the resulting immune response. It is often used to stimulate microglia, which are immune cells found in the brain and spinal cord. Activation of microglia can lead to the production of pro-inflammatory cytokines and other immune mediators, and LPS is often used to stimulate the production of these mediators in order to study their effects on the brain and nervous system.
There are several different LPS strains available, and they can vary in their potency and the specific effects they have on the immune system. It is therefore important to carefully evaluate the literature and consider the specific experimental goals when selecting an LPS strain for use in research.
It is difficult to compare the potency of different LPS strains, as they can vary in their ability to stimulate the immune system depending on the specific cell type, tissue, and species being studied. Some LPS strains are more potent than others in activating the immune system, but this can also depend on the specific immune response being measured.
In general, O111:B4 and O55:B5 are considered to be two of the more potent LPS strains, but it is important to note that different LPS strains can have different effects on the immune system. It is therefore important to carefully evaluate the literature and consider the specific experimental goals when selecting an LPS strain for use in research.
Thank you Mohammad Alzeyadi for such a detailed response! Much appreciated.
Yes, I will be doing a side-by-side comparison.
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