I would like to ask experienced experts: During the submission process of my article, one of the reviewers asked me to provide the histogram results of the statistical analysis of HE staining of internal organs (heart, liver, spleen, lungs and kidneys) between different groups. However, I searched for the relevant literature and some of them just labeled the important structures of the organ structures and then described them, and I did not see the statistical analysis of the HE staining of the organs. So I am looking forward to experienced experts who can give me guidance.
Here are 2 images of the literature I found that I saw no statistical analysis of HE staining in these two papers.2 literature names:
①A preclinical study—systemic evaluation of safety on mesenchymal stem cells derived from human gingiva tissue.
②Acute and sub-acute oral toxicity of Dracaena cinnabari resin methanol extract in rats.
What do you mean by statistical analysis? Are you going to enumerating specific cells or glands, or measuring the thickness of the epithelium, or the height of the cells within the image? All this information between the aggregates can be statistically analyzed and compared. do you mean this?
Khalid Kadhim Hello! What I mean by statistical analysis is that with a certain scoring scale, HE staining can be scored quantitatively or semi-quantitatively to see if there is a difference in HE staining structure of organs between different groups. Do you have a recommendation for a suitable scale?
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