Best protocol for automated cell counting?

Hi all, I am performing an exosome quantification assay and I need to normalize my results to total number of cells. I will not be able to detach my cells from the plate, and therefore cannot use...

17 November 2020 6,583 2 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

11 November 2020 2,478 2 View

Good protocol for metabolite extraction from clostridia?

I am looking for a reliable metabolite extraction protocol for clostridia. Most commonly acetonitrile/methanol/water (2:2:1, v/v/v) is used, however, some research claims that pure methanol is...

26 February 2019 1,126 2 View

How are Refractive index changes controlled?

When doing UV-Vis spectroscopy, how do you control for changes in refractive index? Isn't that something that the UV-Vis machine does automatically?

09 April 2018 8,581 4 View

Where can I find commercial ion selective membranes?

I know there are ion selective electrodes (ISEs) out there for measuring specific ion concentration and these electrodes have an ion selective membrane within them. Where can I purchase the...

02 November 2017 2,924 9 View

Anyone have a good way to quantify total levels of TRIF (Ticam1) using Western blotting or FACS (in murine macrophages)?

18 February 2016 9,107 1 View

Is anyone conducting research dealing with coaching models or reality TV?

I am interested in two types of research topics: One factors involved with successful coaching models used in couples or marriage coaching. Most coaches use Co-Active coaching, GROW or REGROW....

09 January 2015 8,887 3 View

Is anyone conducting research dealing with coaching models or reality TV?

I am interested in two types of research topics: One factors involved with successful coaching models used in couples or marriage coaching. Most coaches use Co-Active coaching, GROW or REGROW....

31 December 2014 9,531 4 View

Can someone please help me understand some aspects of conducting a factor analysis please?

I have collected my data and I am now analyzing it. Here are the areas I cannot understand: 1. The pre-post tests questions are alike every way (except in how participants respond after exposure...

18 October 2014 9,540 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View