03 February 2021 1 3K Report

Hello!

I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the nuclei; as everything seems to be done manually.

I've attached some 20x images of the slides. But I'm concerned about edge detection of the cell and haven't had much luck with segmentation in ImageJ. I lose a lot of edges in-between some of the cells.

  • Does anyone have any other software or ImageJ plugins you'd recommend?
  • Any specific computed histology guides or methods of hepatocyte specific antigens or something similar?
  • Any other guidance?

Thank you for your responses.

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