Hi guys,
I m wondering to know my pAb binding sites to the antigen. I am not sure about my pAb's binding towards N terminal or C terminals of antigen. How I can check this? Also, does molecular weight change based on binding sites (N or C) of Abs?
Can anyone help me?
Thank you in advance!
Shahid
You need to check the antibody's data sheet or ask the manufacturer or supplier. If there is sequence data on what they used for generating the antibody (in case of a peptide antibody) you may use e.g. BLAST or Multalin to align it with your protein's sequence to find out yourself if the information should not be given (usually they do).
Regarding molecular weight, the location of binding does not affect the antibody's MW nor the size of the complex formed with target protein.
Dear Shahid,
Typically, polyclonal antibodies (pAb) contain a large number of different individual antibodies, which all differ somehow in affinity, selectivity and target structure. Compared to a monoclonal antibody (mAb), which may only show affinity to a certain target epitope of the antigen, polyclonal antibodies tend to target all available epitopes on the antigen. Therefore, depending on the used immunogen and the size of the antigen, the polyclonal antibodies are likely to bind all accessible epitopes.
As already pointed out by Wolfgang Schechinger, the mass is not affected by the binding site.
Regards Martin
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