Dear Collegues,
If we want to perform immunohistochemistry /immunofluorence staining from FFPE human biopsy (vein) samples and study the presence of LPS and or common pathogen like Porphyromonas gingivalis in them. Which kind of issues we have to consider to avoid contamination? The samples are sterile but the used reagents are 'normal' commercial immunohistochemistry and immunofluorence materials. Does commercial immunohistochemistry and immunofluorence materials contain LPS residuals and is the cross-contamination (positive control and studied samples) during the process possible?
Very satisfied with your reply :-)
The majority of antibodies for IHC are produced in animals or hybridomas under sterile culture conditions and suspension solutions are filtered to exclude contaminants and have sodium azide to prevent microorganism growth. Check the vendor QC procedures or contact support to learn more about your purchased Ab. Here is an example from Abcam https://www.abcam.com/tag/antibody%20guide
Rabeah Al-Temaimi Thank you. I believe that antibodies are not the problem. I was wondering what about the used other reagents and the protocol itself..
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