Which software do you use for biological FIB-SEM data segmentation?

Dear colleagues, A good FIB-SEM data stack can contain a few thousands pictures. It is very tricky and annoying process to segment them all manually. Which software do you use (real practical...

01 February 2019 2,914 8 View

Which chemical reaction makes HM20 resin pink?

The datasheet says: "After polymerization, the block sometimes turns to a light pink or pink/red color. This seems to be quite normal and should not be a cause for concern. " Indeed, after...

31 December 2018 5,513 6 View

Do you have experience with high pressure freezing (Leica EM PACT2 or ICE) of very soft tissues (testis, etc)?

Dear colleagues, Did you use HPF for some super-soft tissue like testis? Operation with "sampled" specimens like pieces of tissue, small worms, etc. is pretty clear. But when we have some...

09 October 2018 7,461 15 View

Which time between dissection and high pressure freezing is acceptable?

Dear colleagues, Obviously, this time should be as short as possible, but sometimes we need to dissect animals in one building and transfer the samples to another. Which time between dissection...

09 October 2018 6,230 15 View

How do you conduct freeze substitution (Leica AFS) for 6 mm sapphire discs?

Dear colleagues, We have prepared a few 6 mm sapphire discs with cells monolayers by HPF, they are in liquid nitrogen now. These disks are too big for conventional Leica freeze substitution...

05 June 2018 7,979 8 View

I'm looking for a postdoctoral position in microscopy and physics of microorganisms

04 May 2017 1,220 7 View

Does Zika virus have a serious pandemic potential?

31 December 2015 9,104 19 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View