I am looking for a contrast agent specific of plant cell-wall's lignin suited for Scaning Electron Microscopy or X-Ray computed tomography.
Many thanks !
A traditional stain with affinity for wood lignins in EM is KMnO4, potassium permanganate. This has been used in EM since the 1930s. Attached is a link covering some of the available stains for wood in EM.
https://bioresources.cnr.ncsu.edu/resources/transmission-electron-microscopy-for-wood-and-fiber-analysis-a-review/
K and Mn could work for CT; but you should use low kV tube settings....
Tiny KMnO4 concentrations and spacial resolution will be the challenges. In any case you should go for µ-CT...
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