I am planning to perform analysis of liposomes using TEM. Therefore, i thought of using Osmium tetroxide solution as a staining agent.
Are you sure? OsO4 is not a proper agent for NS. Why don't you use common NS agents like uranyl acetate or Phosphotungstic acid?
OsO4 reacts with lipids and proteins providing positive contrast. It is a common reagent for resin embedding preparation, not for NS.
Good luck!
Are you sure? OsO4 is not a proper agent for NS. Why don't you use common NS agents like uranyl acetate or Phosphotungstic acid?
OsO4 reacts with lipids and proteins providing positive contrast. It is a common reagent for resin embedding preparation, not for NS.
Good luck!
Tony Kiio , no worries. Try to use phosphotungstic acid, perhaps it will be easier and faster than order UA.
Good luck!
Don't use osmium tetroxide. It is very toxic!
Use either uranyl acetate (UA), but it is concerned by natural radioactivity, or phosphotungstic acid (PTA).
I'm using neutral PTA, 1% in water pH 7.
You can find a protocol there:
https://www.researchgate.net/post/Hello_I_am_trying_to_visualize_my_liposomes_under_TEM_Can_anyone_help_me_with_details_of_PTA_staining2
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