Dear All
I am preparing mutant HBV from HepG2 cells by transfecting the recombinant plasmid DNA.
Usually, I concentrate HBV by PEG precipitation. But high concentration of PEG affects HBV infection efficiency.
Is there any other/best way to concentrate the HBV which does not affect the infectivity of HBV?
Thank you all in advance.
Regards
Hossain