Dear All

I am preparing mutant HBV from HepG2 cells by transfecting the recombinant plasmid DNA.

Usually, I concentrate HBV by PEG precipitation. But high concentration of PEG affects HBV infection efficiency.

Is there any other/best way to concentrate the HBV which does not affect the infectivity of HBV?

Thank you all in advance.

Regards

Hossain

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