A platform to discuss the methods and technologies involved in DNA sequencing. | Contact experts in DNA Sequencing to get answers
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Questions related to DNA Sequencing
What would be a good program to use to extract enhancer DNA sequences upstream of transcription start site (TSS). For example, I would like to get DNA for a region -3000bp to -6000bp upstream of...
01 January 2015 6,455 4 View
A 'case' sample and a 'control' sample have been sequenced using Illumina platform. After bioinformatic processing, my database contains million of reads, each consisting of 3 nucleotide (codon)...
01 January 2015 5,143 20 View
HCV Evolution
01 January 2015 3,011 4 View
I am currently working with mitochondrial DNA sequences. Thank you very much for your help and time. Regards.
01 January 2015 7,145 2 View
DNA sequencer
01 January 2015 5,058 2 View
If I have about 90% homology with the gene that I did for it DNA sequencing and about 66% homology with protein. It is mean a good results? Thanks
01 January 2015 3,956 6 View
I have DNA sequences from my sample. I want to construct their phylogenetic tree. However, I found more than one stop codon in the middle of the sequences after I translated them to amino acid...
01 January 2015 6,494 4 View
To link the bacterial DNA sequences with immunoinformatics tools and then to get a significant results.
01 January 2015 4,330 1 View
I am searching for fhaB gene sequence of some bordetella pertussis strains for my research but after searching in several databases I could not find that. Can anyone tell me how I can get my...
01 January 2015 8,043 5 View
Does whole exome sequencing add any more information over transcriptome sequencing? I am trying to compare expression profiling in AML cells before and after specific cytokine treatment.
01 January 2015 10,057 3 View
Hi, I would appreciate so much your input to my problem/question. I extracted genomic DNA from yeast for whole genome sequencing (illumina Hiseq technology), and digested the prep with RNAse A....
01 January 2015 7,938 2 View
Is it possible to know whether the DNA samples contain homozygous alleles or heterozygous ones?
12 December 2014 7,389 7 View
I'm going to create contig from meleafris EST datast, searching in est file shows that both there are both poly (A) and poly (T) sequences in EST file. Is it related to the methods used by scientists?
12 December 2014 8,599 3 View
Are there near term benefits that we should be better harvesting? How do we move ahead effectively with sequence data?
12 December 2014 5,976 2 View
I have tried sending my samples to one and it has only been a waste of time. Any suggestions?
12 December 2014 5,141 3 View
Hi all. I have a chromosome segment of roughly 4 Mb that almost does not recombine (barely 1cM overall) but the genetic map was built using another population. The DNA sequence I have from the...
12 December 2014 2,850 6 View
Using PCR, how would you isolate or clone the full genomic copy (all exons included) of a gene from your complex organism of interest starting only with the sequence information of the mRNA for...
12 December 2014 3,388 6 View
I got genomic dna samples and I wonder if need to break the circular strand in order to sequence it. How can I break it? And how can i know if I have circular or linear dna in my samples? Thanks a...
12 December 2014 488 8 View
I am producing DNA aptamers for protein targets and I would like to sequence my enriched aptamer pool using Illumina next generation sequencing techniques. If you know any company providing...
12 December 2014 9,375 2 View
I am working on bactreiophage- I am trying to purify bacteriophage DNA for sequencing using phenol. When I run it still there is smear even when I increase the amount of DNase and RNase.
19 November 2014 6,603 9 View
I would like to buy a next generation DNA sequencer. Any suggestion for a brand that can offer the followings 1. direct sequencing, no PCR needed 2. durable machine, less maintenance cost, 3....
11 November 2014 2,537 9 View
Genomics, DNA Sequencing, molecular genetics, and virology
11 November 2014 7,887 1 View
The scientists already know the DNA sequence of a HIV virus based on which they develop the primers called HIV primers. Now during the PCR they mix the DNA sample of the person with this HIV...
11 November 2014 8,284 7 View
11 November 2014 9,673 7 View