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Questions related to Biochemical Techniques
In the case of liquid samples, the dilution of the liquid sample with diluent is an easy task. But in the case of the solid sample or a powdered sample, it's a bit tedious work. I would like to...
08 August 2017 9,986 11 View
05 August 2017 4,072 9 View
Hi! I am looking for a reverse transcriptase that has no strand displacement but rather 5' to 3' exonuclease activity. The closest thing I found is Bst polymerase from NEB but that is only a weak...
25 July 2017 6,464 2 View
Say, after protein quantification, we mix certain amount of samples, loading dye and reducing reagent, what should be used for bringing up the final volume, deionized H2O or lysis buffer? Is...
20 July 2017 6,430 3 View
Since, we all know, the temperature of Maillard reaction is below 120-180oC. What is the effect of temperatures higher than 180oC on Maillard reaction?
07 July 2017 1,778 3 View
07 July 2017 8,619 3 View
I read many papers saying that they dissolve amyloid beta 25-35 in dd water. I tried to dissolve it at 1mg/ml and later I followed the company's suggestion adding 0.9ul 1N NaOH in the peptide. It...
07 July 2017 3,386 1 View
07 July 2017 9,114 2 View
Hi, I need to check the amount of a certain protein secreted in conditioned media. What I have done so far is plated equal number of cells and then collected equal volume of media. Following this...
06 July 2017 399 3 View
Hello Everyone, the other day I tried to run a ellman's regent experiment using L-cysteine as my control. the overall objective was to plot the values obtained from the standards(L-cysteine) to...
26 June 2017 7,753 6 View
After RT, I dilute the cDNA to tenfold,but the result of ddPCR shows the dilution is not accuracy, it only fivefold. I am confused how that happened? Thank You!
20 June 2017 3,223 3 View
previous I optimised my designed primers without DMSO, but now my subject samples getting problem so I added DMSO, getting good intensity bands along with non specific bands. So now again...
15 June 2017 8,057 1 View
I know that shouldnt be the case but this is what i observe : the [M-H+]- elutes around 6.00 and the [2M-H+]- around 11 minutes in the reversed phase. The spectra look similar and could perfectly...
13 June 2017 1,589 1 View
Hi, I am wondering how to store neutravidin aliquotes. Will the protein be stable after freezing it at -20C and thawing a week later? Thank you, Monika
09 June 2017 4,560 3 View
I am trying to see the expression of some genes, so in my control cell line i get RNA but after i convert it to cDNA i dont see amplification. i am doing quantification of RNA with Nanodrop. The...
06 June 2017 5,861 6 View
06 June 2017 9,426 4 View
06 June 2017 7,361 1 View
I mean using the same DNA for the same pcr conditions you get varying result even when ran at the same time. That is some faint bands while some are bright enough.
06 June 2017 540 3 View
06 June 2017 9,392 7 View
am running an assay of activin a in urine. Could the problem be with my dilution factor?
06 June 2017 7,715 1 View
The last couple batches of RPMI 1640 media I have prepared have not been the correct color after the addition of FBS. When aliquoting small volumes before the addition of FBS, I have the expected...
06 June 2017 1,233 4 View
06 June 2017 7,148 1 View
06 June 2017 6,414 3 View
On the company's advice we're using the Anions 2 kit from Analis, but can't make it work. We see three big peaks close to each other we would like to get further apart. Also even by running single...
05 May 2017 7,865 3 View