A platform to discuss the methods and technologies involved in DNA sequencing. | Contact experts in DNA Sequencing to get answers
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Questions related to DNA Sequencing
After You inject your DNA sequence into the distal gonad region using the microinjection, what happens to the DNA? Is it taken up by the germ line nuclei during the developmeant of the oocyte? If...
27 March 2018 7,519 3 View
I have a putative DNA sequence which has been demonstrated to lie in the proximal promoter of two genes to facilitate their regulation. I am curious if there are approaches (bioinformatics or...
26 March 2018 4,563 3 View
How do I find references to the size of the cDNA sequence I isolated?
13 March 2018 2,224 3 View
I want to make PCoA plots from DNA sequence data. Can anybody give a good hint on the software to use? Alternatively I would take the same advise to make PCoA from binary 0/1 data.
01 March 2018 9,656 6 View
01 March 2018 4,784 3 View
In particular, what do the output datasets look like and what's the error rate ?
12 February 2018 1,742 3 View
Could you categorize the types of diseases according to changes of gene sequence, gene modification, others.
10 February 2018 7,441 1 View
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04 April 2017 5,480 1 View
I want to use Geneland for cytochrome b sequences of the mitochondrial DNA. Could somebody share with me an example input file for DNA sequences?Thank you very much. Raul
21 September 2016 7,705 10 View
SDS, CTAB method already used. at the same time genejet DNA purification kit and combination of mannual and genejet DNA purification kit applied. But each and every time DNA were fragmentated and...
30 July 2016 6,783 4 View
Does anyone know how to draw G-quadruplex by mfold? I tried to draw but it is not result as my expected structure
16 May 2016 2,494 2 View
For the identification of Biosurfactant producing bacteria, I am trying to sequencing the 16s rDNA, that i had done three time. Unfortunately, i did not get any results. After amplification, i...
02 May 2016 7,895 3 View
I have an excel sheet file of both binary (male and female) and continuous characters data, I need to convert it to NEXUS file format, in order to read it with the tree of DNA sequences, for...
07 April 2016 1,385 3 View
Hi! I am asking because I had some luck using the SPAdes assembler to assemble the mitochondrial genome from subsamples of full genomic reads of parasitic nematodes, but it has not been successful...
20 March 2016 5,139 10 View
What are the common primer's (F/R) used to detect bacteriophage endolysins (lysine) and tail holin?
12 November 2015 4,033 1 View
I have tried to propagate phage from single plaque but not able to get titer above 10-7. 10 ml of broth was inoculated with Xanthomonas oryzae culture. When the OD reached 0.2 single plaque...
22 October 2015 244 8 View
I have gram positive and negative bacterias for DNA purification, what low salt buffer I can use to wash cells and keep them intact
17 September 2015 5,082 5 View
We often perform Quikchange mutagenesis on cDNA and send our samples to Genewiz for sequencing. Lately several of us in the same building consistently get "no priming" results for almost all our...
15 June 2015 4,400 7 View
Hello, Do anybody face issues or have concerns about the gRNA scaffold's secondary structure formation. During in-vitro transcription, there is no heating and cooling step to enable the formation...
07 April 2015 8,718 2 View
I am doing DNA extraction from bacteriophage but still i get genomic DNA after adding of both DNase and RNase. so I was wondering is it possible to add DNase after perciptiation with PEG 8000 and...
03 February 2015 916 6 View
See above
02 February 2015 5,811 13 View
I try to obtain sequence of highly variable part noncoding element by sanger sequencing. After polyA/polyT repeat (in direct and reverse sequencing) signal are decreased greatly of interrupted....
02 February 2015 10,031 5 View
Hello everyone I am running a pcr of some genomic DNA and it seems to amplify the genes I looking for fine however when I check the sample in the nanodrop I get a curve like in the picture 260/280...
02 February 2015 3,446 5 View
Which methods can be used?
01 January 2015 5,916 3 View