Which DNA sequences should i select in phylogenetic analysis as i am getting very low bootstrap value for phylogenetic tree using ML method?

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Transfection with CRISPR using turbofectin?

Dear all! we are trying to knockdown a gene in lymphoblastoid cell line using Origene CRISPR/CAS9 kit with the HMR method using the recommended turbofectin reagent. However, we are not getting any...

09 October 2018 8,862 3 View

Single cell isolation post CRISPR/CAS9?

Hi, I performed CRISPR/CAS9 genome editing in HL-60 cell line (suspension culture) to modify a gene of interest for a desired mutation (I used the sgRNA for my gene and a single strand ultramers...

03 April 2018 3,195 2 View

My phylogenetic tree topology is fine but the confidence (nodes bootstrap value) is quite less than the threshold. Do journals accepts such trees?

ML tree HCV NS5B sequences

02 March 2017 7,367 4 View

Dear all ! I am getting the issue of plasmid self ligation during E. Coli transformation every time. How can I minimize the problem?

using pjd301 plasmid, 5UTR insert and DH5-alpha strain

11 December 2016 2,878 7 View

Which region or gene should be the most appropriate for phylogenetic analysis?

Criteria for selection of a gene for phylogeny - let it be the case of HCV ?

04 May 2014 6,532 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View