Hello,DNA sequencing can reflect homozygous or heterozygous.But you should make an amplification of your DNA sample and do the electrophoresis.In this way,you can get the conclusion.Here is a picture of Human`s D1S80 alleles.In the picture below, the stripe with a single light area is homozygous allele ,the ones with two or two above are heterozygous.

Of course. Sequence and look for variation. However, phasing the data may be more complicated.

Sailesh

Yes, it can. Heterozygous allele could be noticed by seeing whether or not half-sized electrophoregram under the lager peak appears.

I think Rudin & Inman's An Introduction to Forensic DNA Analysis (2001) could be the best reference for know-how principle of sequencing and its result.

Best.

Yes of course it does. But if you know the positions phasing the data would be easy else it would be time consuming. Get the assistance of Gel electrophoresis to ensure the presence of your homo/heterozygous alleles.

Accurately, DNA sequencing can detect/idetify the homozygous/heterozygous genotypes of polymorphic sites.

Hi guys ! can someone please explain How can homozygous versus heterozygous be differentiated? on PCR ? I need to figure out patterns for factor V and PT mutations . I suck at this . Help!!

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