A platform to discuss the methods and technologies involved in DNA sequencing. | Contact experts in DNA Sequencing to get answers
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Questions related to DNA Sequencing
I am interested to know is it possible to directly sequence a single gene from total cDNA or gDNA without making genomic or cDNA libraries? Does anyone know about this?
28 January 2019 5,112 7 View
I'm doing a study on mammal colonization timeline in one of the Islands in Tanzania by using mitochondria DNA sequences. Is there a specific analysis which can do that? Your answers will be helpfull.
26 January 2019 4,054 3 View
I have whole genome bisulfite sequencing (WGBS) data for a species without reference genome. Is there any pipeline that I can use to process my data ?
23 January 2019 221 1 View
I am currently interested in the kinetics of the melting DNA, we have some new theory of the double helix melting model, and we want to conduct the experiment to verify it. We want to design a...
15 January 2019 7,588 2 View
Need detailed procedure to align DNA seq. before performing BLAST.
10 January 2019 7,692 3 View
I am trying to locate GREs (glucocorticoid response elements) within a gene sequence. I would like to find a user-friendly online tool to help me find full GRE consensus as well as half sites, if...
09 January 2019 5,681 2 View
I am trying to do some quality control checks on Illumina NGS paired end reads using FastQC 0.11.5. I tried to add the primer sequences from NEBNext multiplex library prep kit to the...
22 December 2018 5,583 1 View
Please help, I'd like to study a interested protein and get all the possible DNA sequences that interacted with the protein, however, I could not get the IP antibody of the protein, so I'd like...
19 December 2018 5,456 3 View
I would like to perform genotype-phenotype-environment association studies along gradients (continuous allele/phenotype change) using low-coverage WGS data (~100 individuals, mean sequencing...
06 December 2018 10,039 4 View
Hello, I have a list of sites (~9,000) that I need to pull out of the mouse genome in a .csv file as below; Name, chromosome, start coordinate, end coordinate, length Does any one know of an...
20 November 2018 9,284 2 View
I am a young scientist who is more or less completely inexperienced when it comes to conducting independent research projects. I have an idea for a research project, and I am planning on aplying...
20 November 2018 7,228 2 View
Hello, I'm using the command line to NCBI BLAST fungal DNA sequences (Table-C1, https://www.ncbi.nlm.nih.gov/books/NBK279684/). The default evalue is 10.0, but one paper I'm following used...
18 November 2018 1,185 3 View
Hello everyone, I have two batches genomic DNA sequences, respectively from ChIP-seq analyses of two species. I know many tools could perform differential enrichment of motifs between samples....
13 November 2018 4,739 3 View
what are the suitable software to analyze of raw DNA sequences for microbial diversity analysis ?
01 November 2018 4,085 3 View
Hi Everyone, I am working on finding the DNA sequence of the proteins with specific function. So far, I got around 5 proteins sequences which show a high similarity (> 96%). I use NCBI to...
22 October 2018 956 3 View
Can anyone suggest free DNA sequence assembler software for Windows ?
19 October 2018 2,510 7 View
I have used Albacore (v2.3.3) to basecall my minION data locally on my machine. Within the produced "workspace" directory albacore is outputing two folders ("pass" and "fail") based on the qscore...
15 October 2018 4,994 4 View
Hi, Currently, I am working on bacterial DNA sequences. I want to calculate summary statistics with mscalc on observed data and simulated data. But how can i convert fasta in ms format? And if i...
15 October 2018 7,449 5 View
Dear colleagues, I pooled together dozens of samples into one library, and sequenced them together. This yielded millions of reads, with the sequencer assigning each unique read-IDs. While the...
09 October 2018 5,673 4 View
Hi all, I am working on a project where we need very high molecular weight DNA for sequencing. We extracted DNA using a Phenol/Chloroform/Isoamyl Alcohol method and then precipitated the DNA...
25 September 2018 3,034 1 View
Sometimes you have to represent a nucleic acid sequence on paper, not as an image, but as a text with notes. Is there any suitable program (Windows OS) possible to divide raw sequence into pieces...
25 September 2018 2,081 2 View
i have nodules from different treated plants of vigna and i want to do molecular characterization of nodule's rhizobium
24 September 2018 5,370 3 View
I need pure phage DNA of my T4 phage for preparing standards for qPCR. I tried 3 different protocols (precipitation with PEG/NaCl, with/without DNase treatment) to prepare T4 phage for DNA...
23 September 2018 5,616 4 View
The way 16S and 18S rDNA sequencing employed for the genetic level identification of bacteria and fungi respectively, am looking for sequence based identification of the plant species targeting...
06 September 2018 5,048 3 View