Transcriptume vs whole exome, which is more informative over the other?

More Mazin Al-Asadi's questions See All

What are the possible ways / instruments that can be used to measure the light uniformity under the concentrator in the lab?

31 December 2018 7,813 0 View

Excuse me, How can we use flow meters to measure the concentrations of mixed gases?

I use the system in the attached file to mix different gases with air, but how can find the concentration of gas in the chamber.

11 December 2018 452 4 View

How to calculate kWh in a PV solar concentrator?

Hi All, I got confused in finding the proper method to calculate the kWh(Energy) that can my solar concentrator produce in a certain location. I 've got the Peak Sun Hours (PSH) calculated which...

08 September 2018 8,584 3 View

Compound Parabolic Concentrator design for solar collector?

I have designed a Compound Parabolic Concentration by using a 3D printer from Formlabs and the shape looks great. I wonder if anyone I wonder if anyone knows where can I get suitable aluminum...

31 December 2016 7,361 5 View

Could anybody help in interpreting an abnormal qPCR melting curve for CD34 primer?

I've got a melting curve showing tow peaks (attached file), a small one before the main one, which is seen in 3 cell lines very similarly while absent in the NTC (no template control). The problem...

06 July 2015 9,625 10 View

How can we reduce the background fluorescence in IF staining of AML cell line cells in suspension?

I am testing translocation of protein from the cytoplasm to the nucleus, I've got a lot of background nuclear fluorescence despite that I used 0.3M glycine in the blocking buffer.

05 June 2014 3,251 7 View

Is there anybody that has experience with p57 expression assay by flow cytometry?

In AML cell lines supposed to show induction of p57 expression after treatment but it showed decrease mean expression with slow proliferation. I used an indirect flow cytometry assay.

01 February 2014 1,377 0 View

The inhibitory effect of Artemisia with nanoparticles on some metal oxides on some bacteria?

I studied for some time the inhibitory effect of Artemisia with nanoparticles on some metal oxides on some bacteria but did not get any result. Can a colleague help you with some advice?

01 January 1970 3,896 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

Mazin Al-Asadi

Thank you Raquel, based on your answer what is the method of choice do you suggest to study the transcriptome expression profile changes? Regards

Korbinian Grote

Hi Mazin, as Raquel already wrote, transcriptome sequencing will get you the expression data, so you will able to generate expression profiles for your two conditions (before / after cytokine treatment). I highly recommend doing replicates if you want proper results. You could then use software like DeSeq2 or edgeR to calculate the differential expression between your two conditions.

Hi Korbinian, many thanks for you kind comment.