Cardiovascular research and its shortcomings?

What are the important cardiovascular research areas? and why is it that after so many decades of prevalent research in this area, it is still the number one cause of death? What are the...

02 March 2016 372 0 View

Cardiac fibroblast specific markers?

I need to check for fibroblasts isolated from rat heart, what all markers are exclusively specific to cardiac fibroblast? I need to do western blot and immunostaining using confocal...

01 February 2016 7,980 2 View

Why does my protein not bind to the DEAE column at pH 7 but it does at pH 8?

the pI of my protein is 5.28. while purifying it when I use buffer of pH 7.2 the protein does not bind to DEAE column but when I use buffer of pH 8 it does. What could be the possible reasons for...

03 April 2015 1,436 15 View

While applying for Ph.D, what all things should we look for and choose the most appropriate lab?

India as well as abroad

11 December 2014 9,218 0 View

How do we go about sequencing data analysis after SDM?

11 December 2014 5,502 1 View

Why do I get no colonies after transformation after SDM?

What could be the reasons for not getting colonies on transformation into E.coli competent after Side directed mutagenesis? There is no problem with DpnI digestion, competent cells, antibiotic...

11 December 2014 6,241 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View