A platform to discuss the methods and technologies involved in DNA sequencing. | Contact experts in DNA Sequencing to get answers
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Questions related to DNA Sequencing
Can someone tell a price estimate (EUR) how much it would cost to sequence a plant genome with an approx size of 1200 Mbp? Platforms: illumina/PacBio/454.
09 September 2014 2,591 9 View
" If either the full ApeKI site (from partial digestion or "chimera formation") or the first 8 bases of common adapter (from ApeKI fragments less than 64 bases) were detected within 64 bases, the...
09 September 2014 1,961 1 View
I am going to be designing DNA oligos for zinc finger sites and I need to avoid edge effects. Are there general principles for the composition of nonspecific flanking sites? I've seen some groups...
08 August 2014 1,962 3 View
The 20 amino acid residue hydropathy values were mapped onto 12 nucleotide-determined values. Instead of averaging 19 amino acids, you average 57 nucleotides. Unfortunately, I published in a...
08 August 2014 1,622 0 View
The same as lacI. I am curious about the when dimer tetramer structure forms in vivo, is it a inducible procedure based on the operator sequence?
08 August 2014 5,117 2 View
Is it possible to migrate negatively charged DNA fragments in capillary electrophoresis using a negative field, i.e. the injector is held at a more negative potential than the sample waste electrode?
08 August 2014 8,670 5 View
What would be the approach to remove repetitive and non-unique sequence motifs/reads with low complexity and increase sequencing capacity with unique genome elements, suitable for "unknown"...
08 August 2014 3,557 3 View
As 16S rDNA sequencing is considered as the common method now used for bacterial identification. Is there any relevance in biochemical tests for identification process.
08 August 2014 7,661 9 View
I have come across certain papers where ITS-2 is claimed as the standard DNA barcode gene. Apart from ITS-2 is there any other gene which can be used? If yes, why so? I am enclosing one such...
08 August 2014 5,750 4 View
I am looking for a publication (preferably a metanalysis) on BRCA1/2 SNPs found in specific ethnicity. Can any one help me with that, especially those working with breast cancer?
08 August 2014 570 3 View
Hi everyone, I am usually accustomed to perform quality trimming on 16S-targeted MiSeq PE reads prior to merging them, however my sequencing facility suggested that they had obtained very good...
08 August 2014 1,485 3 View
We want to install a sequencer machine for DNA in our lab but we are very confused regarding the quality of the machine.
08 August 2014 6,353 5 View
I know that to design a probe for an in situ you need to design an antisense probe that is complimentary to the mRNA of interest. Then, my problem comes when I try to understand how everything is...
08 August 2014 1,392 4 View
Dear friends recently I came to know about the software Q-site finder which find the active sites of protein for drug interaction. Is there any software for the same purpose in DNA too? Depending...
07 July 2014 9,659 3 View
Hello, I want to isolate, amplify and sequence DNA from very small invertebrates (mainly crustaceans) that are colored with rose bengal. The sequencing will be run on an ABI-Prism-3730xl-Sequencer...
07 July 2014 9,161 2 View
I have already amplified rbcl, matk, trnl-f, psba-trnh and ITS regions of my study plants. Now I will be doing sequencing of those regions. I have purified those PCR products by Quaquick gel...
07 July 2014 8,921 5 View
I am using the product for sequencing, I tried to reduce the amount of the template but I got weak bands. I know that we can use gel extraction, but does anyone have suggestion on how to minimize...
07 July 2014 6,288 9 View
I was doing NCBI BLAST for a sequence and I found that in BLAST result it was matching as Reverse & Complimentary. Is it normal that DNA is matching this way? When we sequence the DNA with 27F...
07 July 2014 588 11 View
Cooper et al. (1992) published the first ancient DNA sequences of moa. Is anyone aware of ancient DNA sequences from birds that were published prior to this? Cooper, A., Mourer-Chauvire, C.,...
07 July 2014 4,242 0 View
What are the possible problems with repeat sequence placement on the map and solutions to overcome?
06 June 2014 6,927 0 View
I have FASTQ files as whole genome dataset from illumina I need to extract Viral whole genome for HCV type 4 from my dataset. What can I do?
06 June 2014 4,744 1 View
I have the mRNA sequence for myoglobin in quail. But I cannot figure out how to use this to find the dna sequence for the gene so that I can design primers for it. The BLAST website is still fresh...
06 June 2014 8,937 7 View
It is a new assay for me in multiple SNP analysis with wide case and control range.
06 June 2014 2,796 2 View
I need the steps for obtaining the sequence for tthe XRCC2 gene in human genome from NCBI.
06 June 2014 8,616 3 View