I have a large set of samples to extract DNA from, and am trying to plan out the most efficient way to process them. I have a few options, but the most efficienct would be to first process all of my samples through the extraction and proteinase steps on day 1, store them overnight at 4C, then finish with the cleanup steps on day 2. I'm extracting in 4% SDS with 50mM EDTA, 500mM NaCL, and buffered to pH 8 with 50mM Tris. I use a bead beating step, followed by 15 min 70C incubation, then treat the supernatant with proteinase at 56C for 20 min.
Hi..Robert..
I think yes
DNA will stay stable in this 4C because it need more Temp.to be denaturat.
I have done it several times and still, I got good quality DNA. Yes, it works.
Even after DNA extraction, its stable even at RT. But better to maintain low temperature to minimize DNAse activity.
Hello!
4% SDS prevents any DNAse activity. DNA in this solution is going to be stable even at room temperature for a long period of time.
Use EDTA fir the extraction buffer , its good chelating agent inactivated activity of endonuckeases.
İYİ GÜNLER
Proteinaz muamelesinden sonra stabil kalmayabilir diye düşünüyorum kolay gelsin
Thanks so much for the feedback all, good to hear that others have successfully tried this
Yes , DNA Should be dissolved in TE buffer or neuclease free water and store 4 degree ir -20.
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