How we can improve CfDNA concentration through extraction?. Can we use Thermocycler or precipitation to concentrate CfDNA without damaging DNA integrity?.
Hi! We use Vivacon 500 spin columns with 30,000 MWCO Hydrosart membrane (VivaProducts) for cfDNA when we need to concentrate cfDNA and experience relatively small losses, about 10%. We quantified with qPCR the amplifiable molecules both before and after concentration. Please see our paper for details ("Considerations and quality controls when analyzing cell-free tumor DNA") and be aware of the risk of sample inhibition when you concentrate, depending on your extraction method.