We have to select plasma or serum to collect in order to measure mRNA levels of certain genes.
Hi all, I am interested in whether the strength of correlation estimates between personality & depression scores is equally strong on two measurement occasions - when adolescents are 13yrs old...
30 September 2022 8,942 5 View
Hi! I am trying to understand how personality traits change in adolescents over time (2 measurement occasions with the same individuals). I am using linear mixed models in SPSS for that purpose....
14 April 2022 3,808 5 View
Hello all, I am in the process of writing a lab report about two lab exercises we just had at uni. The first one was a PCR of a DNA fragment, containing one of three variations of the CFTR gene,...
10 December 2020 4,572 2 View
Hi everyone, I am in the very initial stages of planning an RNA sequencing experiment. I want to find out the differential gene expression in my bacterial strain of interest when exposed to...
12 June 2020 6,053 3 View
For my dissertation I would like to analyse handwritten letters in English from Spanish (secondary school) students. I would receive them from a school in Spain but all of a sudden it is uncertain...
11 May 2017 8,193 3 View
Did anybody have an issue of not picking up variant in Nested fragment in one PCR conditions and picking it up in the other? By PCR conditions I mean different Tag Polymerase used. The variant...
01 February 2016 2,696 5 View
I am using LR-PCR fragments as a template for Nested PCR. After performing Nested PCR and sequencing on one of the LR-PCR products I observe low intensity substitutions and deletions which do not...
11 November 2015 217 3 View
Is it possible to do a model of anti-inflammatory activity of a selected phytochemical? Is QSAR applicable?
01 January 1970 2,446 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View